Product Description |
Recombinant Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe42-Tyr995(ITGA5) acidic tail&Gln21-Asp728(ITGB1) basic tail. |
Product Background |
Integrin alpha 5/ beta 1, also known as VLA-5, is a widely expressed non-covalent heterodimer of a 160 kDa alpha 5 and a 130 kDa beta 1 Integrin subunit.Alpha 5/ beta 1 is up‑regulated on tumor vasculature and promotes angiogenesis. |
Product Category |
Recombinant Protein |
Target |
Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer |
Synonyms |
Integrin alpha 5 beta 1; VLA-5; ITGA5&ITGB1; Integrin alpha 5 β 1; α5β1 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Phe42-Tyr995(ITGA5) acidic tail & Gln21-Asp728(ITGB1) basic tail |
Molecular Weight |
The protein has a predicted MW of 111.8 kDa (ITGA5) & 83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 110-140 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized 0.5ug Anti-ITGA5&ITGB1 Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human ITGA5&ITGB1, His Tag with the EC50 of 55.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized 0.5ug Anti-ITGA5&ITGB1 Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human ITGA5&ITGB1, His Tag with the EC50 of 55.3ng/ml determined by ELISA. |
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Biotinylated Human ITGA5&ITGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human ITGA5&ITGB1 is greater than 90% as determined by SEC-HPLC. |
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