Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein (ITG-HM451B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized 0.5_g Anti-ITGA5&ITGB1 Antibody, hFc Tag at 0.5_g/ml (100_l/Well) on the plate. Dose response curve for Biotinylated Human ITGA5&ITGB1, His Tag with the EC50 of 55.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Phe42-Tyr995(ITGA5)acidic tail & Gln21-Asp728(ITGB1)basic tail

Catalog: ITG-HM451B

$750.00
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Product Description Recombinant Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Phe42-Tyr995(ITGA5) acidic tail&Gln21-Asp728(ITGB1) basic tail.
Product Category Recombinant Protein
Target Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer
Gene/Protein ID P08648(ITGA5)&P05556-1(ITGB1)
Species Human
Exact Sequence Phe42-Tyr995(ITGA5)acidic tail & Gln21-Asp728(ITGB1)basic tail
Molecular Weight The protein has a predicted MW of 111.8 kDa (ITGA5)&83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 110-140 kDa based on Tris-Bis PAGE result.
Activity Immobilized 0.5_g Anti-ITGA5&ITGB1 Antibody, hFc Tag at 0.5_g/ml (100_l/Well) on the plate. Dose response curve for Biotinylated Human ITGA5&ITGB1, His Tag with the EC50 of 55.3ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized 0.5_g Anti-ITGA5&ITGB1 Antibody, hFc Tag at 0.5_g/ml (100_l/Well) on the plate. Dose response curve for Biotinylated Human ITGA5&ITGB1, His Tag with the EC50 of 55.3ng/ml determined by ELISA.
The purity of Biotinylated Human ITGA5&ITGB1 is greater than 90% as determined by SEC-HPLC.
Biotinylated Human ITGA5&ITGB1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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