Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein (ITG-HM451B)
Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein (ITG-HM451B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Biotinylated Human ITGA5&ITGB1, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-ITGA5&ITGB1 Antibody, hFc Tag with the EC50 of 30.7ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Phe42-Tyr995(ITGA5) acidic tail & Gln21-Asp728(ITGB1) basic tail
Catalog #ITG-HM451B
Regular price $660.00
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Product Description

Recombinant Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus of ITGA5.It contains Phe42-Tyr995(ITGA5) acidic tail&Gln21-Asp728(ITGB1) basic tail.

Product Background

Integrin alpha 5/ beta 1, also known as VLA-5, is a widely expressed non-covalent heterodimer of a 160 kDa alpha 5 and a 130 kDa beta 1 Integrin subunit.Alpha 5/ beta 1 is up‑regulated on tumor vasculature and promotes angiogenesis.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer

Synonyms

Integrin alpha 5 beta 1; VLA-5; ITGA5&ITGB1; Integrin alpha 5 β 1; α5β1

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Phe42-Tyr995(ITGA5) acidic tail & Gln21-Asp728(ITGB1) basic tail

Molecular Weight

The protein has a predicted MW of 111.8 kDa (ITGA5) & 83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 110-140 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human ITGA5&ITGB1, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-ITGA5&ITGB1 Antibody, hFc Tag with the EC50 of 30.7ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human ITGA5&ITGB1, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-ITGA5&ITGB1 Antibody, hFc Tag with the EC50 of 30.7ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human ITGA5&ITGB1, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-ITGA5&ITGB1 Antibody, hFc Tag with the EC50 of 30.7ng/ml determined by ELISA (QC Test).
Biotinylated Human ITGA5&ITGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human ITGA5&ITGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human ITGA5&ITGB1 is greater than 90% as determined by SEC-HPLC. The purity of Biotinylated Human ITGA5&ITGB1 is greater than 90% as determined by SEC-HPLC.

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