Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein (ITG-HM451B)
Catalog: ITG-HM451B
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| Product Description | Recombinant Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Phe42-Tyr995(ITGA5) acidic tail&Gln21-Asp728(ITGB1) basic tail. |
| Product Category | Recombinant Protein |
| Target | Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer |
| Gene/Protein ID | P08648(ITGA5)&P05556-1(ITGB1) |
| Species | Human |
| Exact Sequence | Phe42-Tyr995(ITGA5)acidic tail & Gln21-Asp728(ITGB1)basic tail |
| Molecular Weight | The protein has a predicted MW of 111.8 kDa (ITGA5)&83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 110-140 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized 0.5_g Anti-ITGA5&ITGB1 Antibody, hFc Tag at 0.5_g/ml (100_l/Well) on the plate. Dose response curve for Biotinylated Human ITGA5&ITGB1, His Tag with the EC50 of 55.3ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC |
| Endotoxin | Less than 1EU per _g by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized 0.5_g Anti-ITGA5&ITGB1 Antibody, hFc Tag at 0.5_g/ml (100_l/Well) on the plate. Dose response curve for Biotinylated Human ITGA5&ITGB1, His Tag with the EC50 of 55.3ng/ml determined by ELISA. |
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The purity of Biotinylated Human ITGA5&ITGB1 is greater than 90% as determined by SEC-HPLC. |
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Biotinylated Human ITGA5&ITGB1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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