Name: Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein (ITG-HM451B)
Brand: KactusBio
SKU: ITG-HM451B-100UG
Price: 660.0 USD
Availability: InStock

Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein (ITG-HM451B)

Immobilized Biotinylated Human ITGA5&ITGB1, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-ITGA5&ITGB1 Antibody, hFc Tag with the EC50 of 30.7ng/ml determined by ELISA (QC Test).

Biotinylated Human ITGA5&ITGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human ITGA5&ITGB1 is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Biotinylated Human ITGA5&ITGB1, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-ITGA5&ITGB1 Antibody, hFc Tag with the EC50 of 30.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Phe42-Tyr995(ITGA5) acidic tail & Gln21-Asp728(ITGB1) basic tail

Catalog #ITG-HM451B

Size

100UG

500UG

Order now, ships by:

[{"id":42885089296606,"title":"100UG","option1":"100UG","option2":null,"option3":null,"sku":"ITG-HM451B-100UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Biotinylated Human Integrin alpha 5 beta 1 (ITGA5\u0026ITGB1) Heterodimer Protein (ITG-HM451B) - 100UG","public_title":"100UG","options":["100UG"],"price":66000,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}},{"id":43584144081118,"title":"500UG","option1":"500UG","option2":null,"option3":null,"sku":"ITG-HM451B-500UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Biotinylated Human Integrin alpha 5 beta 1 (ITGA5\u0026ITGB1) Heterodimer Protein (ITG-HM451B) - 500UG","public_title":"500UG","options":["500UG"],"price":220000,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}}]

Product Description	Recombinant Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus of ITGA5.It contains Phe42-Tyr995(ITGA5) acidic tail&Gln21-Asp728(ITGB1) basic tail.
Product Background	Integrin alpha 5/ beta 1, also known as VLA-5, is a widely expressed non-covalent heterodimer of a 160 kDa alpha 5 and a 130 kDa beta 1 Integrin subunit.Alpha 5/ beta 1 is up‑regulated on tumor vasculature and promotes angiogenesis.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer
Synonyms	Integrin alpha 5 beta 1; VLA-5; ITGA5&ITGB1; Integrin alpha 5 β 1; α5β1
Accession	P08648(ITGA5)&P05556-1(ITGB1)
Species	Human
Biotinylated	yes
Amino Acid Range	Phe42-Tyr995(ITGA5) acidic tail & Gln21-Asp728(ITGB1) basic tail
Molecular Weight	The protein has a predicted MW of 111.8 kDa (ITGA5) & 83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 110-140 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human ITGA5&ITGB1, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-ITGA5&ITGB1 Antibody, hFc Tag with the EC50 of 30.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human ITGA5&ITGB1, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-ITGA5&ITGB1 Antibody, hFc Tag with the EC50 of 30.7ng/ml determined by ELISA (QC Test).
	Biotinylated Human ITGA5&ITGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human ITGA5&ITGB1 is greater than 90% as determined by SEC-HPLC.