• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Biotinylated Human ITGAV&ITGB1, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ITGAV Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA.
  • Amino Acid Range: Phe31-Val992 (ITGAV) acidic tail & Gln21-Asp728 (ITGB1) basic tail
Catalog #ITG-HM4V1B
Price
Regular price $660.00
Regular price Sale price $660.00
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein (ITG-HM4V1B) Summary

Product Description

 Recombinant Biotinylated Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein is expressed as a heterodimer of ITGAV and ITGB1 by co-expression in HEK293. Subunit ITGAV is fused with a His tag and a Avi tag at the C-terminus and subunit ITGB1 contains no tag.It contains Phe31-Val992 (ITGAV) acidic tail and Gln21-Asp728 (ITGB1) basic tail.

Product Background

 Integrins are transmembrane heterodimeric receptors that contribute to diverse biological functions and play a conserved role in mediating cell adhesion to fixed extracellular ligands and in the maintenance of tissue integrity. αVβ1 is the major integrin on several different primary fibroblasts responsible for binding to TGFb1 LAP and for mediating activation of latent TGFb by these cells.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Biotinylated Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein (ITG-HM4V1B) Specifications/Details

Protein
 Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer
Synonyms
 ITGAV&ITGB1; αVβ1; Integrin alpha V beta 1; Integrin alpha V β 1
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Phe31-Val992 (ITGAV) acidic tail & Gln21-Asp728 (ITGB1) basic tail
Molecular Weight
 The protein has a predicted MW of 114 kDa (ITGAV) and 83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 140-170 kDa (ITGAV) and 105-135 kDa (ITGB1) based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human ITGAV&ITGB1, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ITGAV Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA.
Product Tag
 C-His-Avi

Fluorophore

 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS, 8% trehalose (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human ITGAV&ITGB1, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ITGAV Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA. Immobilized Biotinylated Human ITGAV&ITGB1, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ITGAV Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA.
Biotinylated Human ITGAV&ITGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human ITGAV&ITGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human ITGAV&ITGB1 is greater than 90% as determined by SEC-HPLC. The purity of Biotinylated Human ITGAV&ITGB1 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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