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Biotinylated Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein (ITG-HM4V1B) Summary
Product Description |
Recombinant Biotinylated Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein is expressed as a heterodimer of ITGAV and ITGB1 by co-expression in HEK293. Subunit ITGAV is fused with a His tag and a Avi tag at the C-terminus and subunit ITGB1 contains no tag.It contains Phe31-Val992 (ITGAV) acidic tail and Gln21-Asp728 (ITGB1) basic tail. |
Product Background |
Integrins are transmembrane heterodimeric receptors that contribute to diverse biological functions and play a conserved role in mediating cell adhesion to fixed extracellular ligands and in the maintenance of tissue integrity. αVβ1 is the major integrin on several different primary fibroblasts responsible for binding to TGFb1 LAP and for mediating activation of latent TGFb by these cells. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Biotinylated Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein (ITG-HM4V1B) Specifications/Details
Protein |
Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer |
Synonyms |
ITGAV&ITGB1; αVβ1; Integrin alpha V beta 1; Integrin alpha V β 1 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Phe31-Val992 (ITGAV) acidic tail & Gln21-Asp728 (ITGB1) basic tail |
Molecular Weight |
The protein has a predicted MW of 114 kDa (ITGAV) and 83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 140-170 kDa (ITGAV) and 105-135 kDa (ITGB1) based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human ITGAV&ITGB1, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ITGAV Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 8% trehalose (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human ITGAV&ITGB1, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ITGAV Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA. |
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Biotinylated Human ITGAV&ITGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human ITGAV&ITGB1 is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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