Biotinylated Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein (ITG-HM4V3B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human ITGAV&ITGB3, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Phe31-Val992(ITGAV) acidic tail & Gly27-Asp718(ITGB3) basic tail

Catalog: ITG-HM4V3B

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Product Description Recombinant Biotinylated Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe31-Val992 (ITGAV) acidic tail & Gly27-Asp718 (ITGB3) basic tail.

Product Background

ITGAV&ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV&ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling. ITGAV&ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling. ITGAV&ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling. ITGAV&ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling.

Product Category

Recombinant Protein

Target

ITGAV&ITGB3

Gene/Protein ID

P06756-1(ITGAV)&P05106-1(ITGB3)

Species

Human

Exact Sequence

Phe31-Val992(ITGAV) acidic tail & Gly27-Asp718(ITGB3) basic tail
Molecular Weight The protein has a predicted MW of 114 kDa (ITGAV) & 81.3 kDa (ITGB3). Due to glycosylation, the protein migrates to 100-160 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Biotinylated Human ITGAV&ITGB3, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human ITGAV&ITGB3, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
The purity of Biotinylated Human ITGAV&ITGB3 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human ITGAV&ITGB3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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