Biotinylated Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein (ITG-HM4V3B)
Catalog: ITG-HM4V3B
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| Product Description | Recombinant Biotinylated Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Phe31-Val992(ITGAV) acidic tail&Gly27-Asp718(ITGB3) basic tail. |
| Product Category | Recombinant Protein |
| Target | ITGAV&ITGB3 |
| Gene/Protein ID | P06756-1(ITGAV)&P05106-1(ITGB3) |
| Species | Human |
| Exact Sequence | Phe31-Val992(ITGAV)acidic tail & Gly27-Asp718(ITGB3)basic tail |
| Molecular Weight | The protein has a predicted MW of 114 kDa (ITGAV)&81.3 kDa (ITGB3). Due to glycosylation, the protein migrates to 90-145 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Anti-alpha V beta3 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB3, His Tag with the EC50 of 54ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-alpha V beta3 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB3, His Tag with the EC50 of 54ng/ml determined by ELISA. |
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The purity of Biotinylated Human ITGAV&ITGB3 is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human ITGAV&ITGB3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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