Biotinylated Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein (ITG-HM4V3B)
Biotinylated Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein (ITG-HM4V3B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human ITGAV&ITGB3, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Phe31-Val992(ITGAV) acidic tail & Gly27-Asp718(ITGB3) basic tail
Catalog #ITG-HM4V3B
Regular price $600.00
/
Size

Order now, ships by:

Product Description
Recombinant Biotinylated Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe31-Val992 (ITGAV) acidic tail & Gly27-Asp718 (ITGB3) basic tail.
Product Background
ITGAV&ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV&ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling. ITGAV&ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling. ITGAV&ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling. ITGAV&ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling.
Product Category
Recombinant Protein
Target
ITGAV&ITGB3
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Phe31-Val992(ITGAV) acidic tail & Gly27-Asp718(ITGB3) basic tail
Molecular Weight
The protein has a predicted MW of 114 kDa (ITGAV) & 81.3 kDa (ITGB3). Due to glycosylation, the protein migrates to 100-160 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human ITGAV&ITGB3, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human ITGAV&ITGB3, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
Biotinylated Human ITGAV&ITGB3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human ITGAV&ITGB3 is greater than 95% as determined by SEC-HPLC.

Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recently viewed