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Biotinylated Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein, Ultra Low Endotoxin (ITG-HM4V3B-UL) Summary
Product Description |
Recombinant Biotinylated Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus of ITGAV.It contains Phe31-Val992 (ITGAV) acidic tail and Gly27-Asp718 (ITGB3) basic tail. |
Product Background |
ITGAV&ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV&ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling. ITGAV&ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling. ITGAV&ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling. ITGAV&ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Biotinylated Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein, Ultra Low Endotoxin (ITG-HM4V3B-UL) Specifications/Details
Protein |
Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer |
Synonyms |
αVβ3; Integrin alpha V beta 3; beta3; alphaV; ITGAV&ITGB3;Integrin alpha V β 3; β3 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Phe31-Val992(ITGAV) acidic tail & Gly27-Asp718(ITGB3) basic tail |
Molecular Weight |
The protein has a predicted MW of 114 kDa (ITGAV) & 81.3 kDa (ITGB3). Due to glycosylation, the protein migrates to 100-160 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human ITGAV&ITGB3, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 0.64ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human ITGAV&ITGB3, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 0.64ng/ml determined by ELISA. |
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Biotinylated Human ITGAV&ITGB3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human ITGAV&ITGB3 is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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