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Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein, Ultra Low Endotoxin (ITG-HM4V8B-UL) Summary
Product Description |
Recombinant Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus of ITGAV.It contains Phe31-Val992(ITGAV) acidic tail and Glu43-Arg684(ITGB8) basic tail. |
Product Background |
Deletions of the genes encoding the integrin αVβ8 (Itgav, Itgb8) have been shown to result in abnormal vascular development in the CNS, including prenatal and perinatal hemorrhage. Other work has indicated that a major function of this integrin in vivo is to promote TGFβ activation. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein, Ultra Low Endotoxin (ITG-HM4V8B-UL) Specifications/Details
Protein |
Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer |
Synonyms |
αVβ8; Integrin alpha V beta 8; TGAV&ITGB8;Integrin alpha V β 8 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Phe31-Val992(ITGAV) acidic tail & Glu43-Arg684(ITGB8) basic tail |
Molecular Weight |
The protein has a predicted MW of 114 kDa (ITGAV) and 76 kDa (ITGB8). Due to glycosylation, the protein migrates to 85-145 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human ITGAV&ITGB8, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human ITGAV&ITGB8, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA. |
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Immobilized Human Latent TGF beta 1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB8, His Tag with the EC50 of 9.62ug/ml determined by ELISA. |
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Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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