Biotinylated Human Kallikrein 2/KLK2 Protein (active form) (KLK-HM402B

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 85% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human Kallikrein 2, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Kallikrein 2 Antibody, hFc Tag with the EC50 of 28.0ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate: PFR-AMC. The specific activity is >450 pmol/min/µg.
Amino Acid Range: Ile25-Pro261

Catalog #KLK-HM402B

Price

$675⁰⁰

Size

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Biotinylated Human Kallikrein 2/KLK2 Protein (active form) (KLK-HM402B) Summary

Product Description	Recombinant Biotinylated Human Kallikrein 2/KLK2 Protein (active form) is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ile25-Pro261.
Product Background	Kallikrein-related peptidase 2 (KLK2) is a serine protease exhibiting antiangiogenic properties through proteolytic activity. KLK2 is overexpressed in prostate cancer and plays a pivotal role in cancer progression, establishing it as a potential therapeutic target.

Protein	Kallikrein 2/KLK2
Synonyms	KLK2; Kallikrein 2; hGK-1; hK2; KLK2A2;hGK1
Accession	P20151-1
Species	Human
Biotinylated	yes
Amino Acid Range	Ile25-Pro261
Molecular Weight	The protein has a predicted MW of 29.21 kDa. Due to glycosylation, the protein migrates to 32-38 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human Kallikrein 2, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Kallikrein 2 Antibody, hFc Tag with the EC50 of 28.0ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate: PFR-AMC. The specific activity is >450 pmol/min/µg.
Product Tag	C-His-Avi
Fluorophore	Biotin-labeled
Expression System	HEK293
Purity	> 85% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 20mM Tris, 150mM NaCl, 8% trehalose, 0.05% Brij-35, 5mM Benzamidine (pH 8.0).
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human Kallikrein 2, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Kallikrein 2 Antibody, hFc Tag with the EC50 of 28.0ng/ml determined by ELISA.
	Biotinylated Human Kallikrein 2 on Bis-Tris PAGE under reduced condition. The purity is greater than 85%.
	Loaded Biotinylated Human Kallikrein 2, His-Avi Tag on Streptavidin-Biosensor can bind Anti-Kallikrein 2 Antibody, hFc Tag with an affinity constant of 0.24 nM as determined in BLI assay.
	The purity of Biotinylated Human Kallikrein 2 is greater than 95% as determined by SEC-HPLC.

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