Biotinylated Human Kallikrein 3/PSA-Protein (active form) (Primary Ami

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human Human Kallikrein 3, No Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Kallikrein 3 Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide (Suc-RPY-pNA). The specific activity is >500 pmol/min/ug.
Amino Acid Range: Ile25-Pro261

Catalog #KLK-HM003B

Price

$578⁰⁰

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Biotinylated Human Kallikrein 3/PSA-Protein (active form) (Primary Amine Labeling) (KLK-HM003B) Summary

Product Description	Recombinant Biotinylated Human Kallikrein 3/PSA-Protein (active form) is expressed from HEK293 without tag.It contains Ile25-Pro261.
Product Background	Kallikrein-related peptidase 3 (KLK3), also known as prostate-specific antigen (PSA), is the most useful biomarker for prostate cancer (PCa). KLK3 is suggested to play a role in regulating cancer growth through anti-angiogenic activity in vivo and in vitro.

Protein	Kallikrein 3/PSA
Synonyms	PSA; Kallikrein 3; Kallikrein-3; P-30 antigen; Semenogelase; KLK3; APS
Accession	P07288-1
Species	Human
Biotinylated	yes
Amino Acid Range	Ile25-Pro261
Molecular Weight	The protein has a predicted MW of 26.08 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human Human Kallikrein 3, No Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Kallikrein 3 Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide (Suc-RPY-pNA). The specific activity is >500 pmol/min/ug.
Product Tag	No Tag
Fluorophore	Biotin-labeled
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 50mM Tris, 150mM NaCl, 0.05% Brij-35 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human Human Kallikrein 3, No Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Kallikrein 3 Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA.
	Biotinylated Human Kallikrein 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human Kallikrein 3 is greater than 95% as determined by SEC-HPLC.