Name: Biotinylated Human Kallikrein 5/KLK5 Protein (active form) (KLK-HM405B)
Brand: KactusBio
SKU: KLK-HM405B-100UG
Price: 578.0 USD
Availability: InStock

Biotinylated Human Kallikrein 5/KLK5 Protein (active form) (KLK-HM405B)

Immobilized Biotinylated Human Kallikrein 5, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA.

Biotinylated Human Kallikrein 5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Anti-Kallikrein 5 Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human Kallikrein 5, His-Avi Tag with an affinity constant of 1.67 nM as determined in BLI assay (Gator® Prime).

The purity of Biotinylated Human Kallikrein 5 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human Kallikrein 5, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >400 pmol/min/µg.
Amino Acid Range: Ile67-Ser293

Catalog #KLK-HM405B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human Kallikrein 5/KLK5 Protein (active form) (KLK-HM405B) Summary

Product Description	Recombinant Biotinylated Human Kallikrein 5/KLK5 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ile67-Ser293.
Product Background	The inhibition of kallikrein 5 (KLK5) has been identified as a potential strategy for treatment of the genetic skin disorder Netherton syndrome, in which loss-of-function mutations in the SPINK5 gene lead to down-regulation of the endogenous inhibitor LEKTI-1 and profound skin-barrier defects with severe allergic manifestations. To aid in the development of a medicine for this target, an X-ray crystallographic system was developed to facilitate fragment-guided chemistry and knowledge-based drug-discovery approaches.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human Kallikrein 5/KLK5 Protein (active form) (KLK-HM405B) Specifications/Details

Protein	Kallikrein 5/KLK5
Synonyms	Kallikrein c; Klnc; KLK5; KLKL2; KLK-L2; SCTE
Accession	Q9Y337
Species	Human
Biotinylated	yes
Amino Acid Range	Ile67-Ser293
Molecular Weight	The protein has a predicted MW of 32.51 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human Kallikrein 5, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >400 pmol/min/µg.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 50mM MES, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in 50mM MES, 150mM NaCl (pH 5.5). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human Kallikrein 5, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA.
	Biotinylated Human Kallikrein 5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Anti-Kallikrein 5 Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human Kallikrein 5, His-Avi Tag with an affinity constant of 1.67 nM as determined in BLI assay (Gator® Prime).
	The purity of Biotinylated Human Kallikrein 5 is greater than 95% as determined by SEC-HPLC.