Regular price
$578.00
Order by 4pm ET for delivery on:
Biotinylated Human Kallikrein 5/KLK5 Protein (active form) (KLK-HM405B) Summary
Product Description |
Recombinant Biotinylated Human Kallikrein 5/KLK5 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ile67-Ser293. |
Product Background |
The inhibition of kallikrein 5 (KLK5) has been identified as a potential strategy for treatment of the genetic skin disorder Netherton syndrome, in which loss-of-function mutations in the SPINK5 gene lead to down-regulation of the endogenous inhibitor LEKTI-1 and profound skin-barrier defects with severe allergic manifestations. To aid in the development of a medicine for this target, an X-ray crystallographic system was developed to facilitate fragment-guided chemistry and knowledge-based drug-discovery approaches. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Biotinylated Human Kallikrein 5/KLK5 Protein (active form) (KLK-HM405B) Specifications/Details
Protein |
Kallikrein 5/KLK5 |
Synonyms |
Kallikrein c; Klnc; KLK5; KLKL2; KLK-L2; SCTE |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ile67-Ser293 |
Molecular Weight |
The protein has a predicted MW of 32.51 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human Kallikrein 5, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >400 pmol/min/µg. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 50mM MES, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in 50mM MES (pH 5.5). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Biotinylated Human Kallikrein 5, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA. |
|
|
Biotinylated Human Kallikrein 5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Biotinylated Human Kallikrein 5 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
