Name: Biotinylated Human Kallikrein 7/KLK7 Protein (pro form) (KLK-HM417B)
Brand: KactusBio
SKU: KLK-HM417B-100UG
Price: 578.0 USD
Availability: InStock

Biotinylated Human Kallikrein 7/KLK7 Protein (pro form) (KLK-HM417B)

Immobilized Biotinylated Human Kallikrein 7, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Kallikrein 7 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA.

Biotinylated Human Kallikrein 7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human Kallikrein 7 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human Kallikrein 7, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Kallikrein 7 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is >150 pmol/min/µg.
Amino Acid Range: Glu23-Arg253

Catalog #KLK-HM417B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human Kallikrein 7/KLK7 Protein (pro form) (KLK-HM417B) Summary

Product Description	Recombinant Biotinylated Human Kallikrein 7/KLK7 Protein (pro form) is expressed from HEK293 with His tag and Avi Tag at the C-terminus. The protein needs to be activated by Thermolysin for an activated form.It contains Glu23-Arg253.
Product Background	Kallikrein 7 (KLK7) is a secreted serine protease with chymotrypsic protease activity. Abnormally high expression of KLK7 is closely related to the occurrence and development of various types of cancer. Therefore, KLK7 has been identified as a potential target for cancer drug development design in recent years. KLK7 mediates various biological and pathological processes in tumorigenesis, including cell proliferation, migration, invasion, angiogenesis, and cell metabolism, by hydrolyzing a series of substrates such as membrane proteins, extracellular matrix proteins, and cytokines.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human Kallikrein 7/KLK7 Protein (pro form) (KLK-HM417B) Specifications/Details

Protein	Kallikrein 7/KLK7
Synonyms	Kallikrein-7; hK7; Serine protease 6; hSCCE; KLK7; PRSS6; SCCE
Accession	P49862
Species	Human
Biotinylated	yes
Amino Acid Range	Glu23-Arg253
Molecular Weight	The protein has a predicted MW of 28.11 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human Kallikrein 7, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Kallikrein 7 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is >150 pmol/min/µg.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 20mM HEPES, 150mM NaCL (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human Kallikrein 7, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Kallikrein 7 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA.
	Biotinylated Human Kallikrein 7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human Kallikrein 7 is greater than 95% as determined by SEC-HPLC.