Product Description |
Recombinant Biotinylated Human Kallikrein 7/KLK7 Protein (pro form) is expressed from HEK293 with His tag and Avi Tag at the C-terminus. The protein needs to be activated by Thermolysin for an activated form.It contains Glu23-Arg253. |
Product Background |
Kallikrein 7 (KLK7) is a secreted serine protease with chymotrypsic protease activity. Abnormally high expression of KLK7 is closely related to the occurrence and development of various types of cancer. Therefore, KLK7 has been identified as a potential target for cancer drug development design in recent years. KLK7 mediates various biological and pathological processes in tumorigenesis, including cell proliferation, migration, invasion, angiogenesis, and cell metabolism, by hydrolyzing a series of substrates such as membrane proteins, extracellular matrix proteins, and cytokines. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
Kallikrein 7/KLK7 |
Synonyms |
Kallikrein-7; hK7; Serine protease 6; hSCCE; KLK7; PRSS6; SCCE |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Glu23-Arg253 |
Molecular Weight |
The protein has a predicted MW of 28.11 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 20mM HEPES, 150mM NaCL (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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