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Biotinylated Human KIR3DL3 Protein (KR3-HM4L3B) Summary
Product Description |
Recombinant Biotinylated Human KIR3DL3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu26-Leu322. |
Product Background |
Killer-cell immunoglobulin-like receptor (KIR) 3DL3 is a framework gene present in all human KIR haplotypes.KIR3DL3 has been shown to be constitutively expressed at a low RNA level in peripheral blood mononuclear cell (PBMC) and decidual natural kill (NK) cells. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Biotinylated Human KIR3DL3 Protein (KR3-HM4L3B) Specifications/Details
Protein |
KIR3DL3 |
Synonyms |
CD158Z; KIR2DS2; KIR3DL7; KIR44; KIRC1; KIR3DL3 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Glu26-Leu322 |
Molecular Weight |
The protein has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.254 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM NaCl, 0.2M L-arginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human B7-H7, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human KIR3DL3, His-Avi Tag with an affinity constant of 0.254 uM as determined in SPR assay (Biacore T200). |
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Biotinylated Human KIR3DL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human KIR3DL3 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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