Biotinylated Human KIR3DL3 Protein (KR3-HM4L3B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.254 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Glu26-Leu322
Catalog #KR3-HM4L3B
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Product Description

 Recombinant Biotinylated Human KIR3DL3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu26-Leu322.

Product Background

 Killer-cell immunoglobulin-like receptor (KIR) 3DL3 is a framework gene present in all human KIR haplotypes.KIR3DL3 has been shown to be constitutively expressed at a low RNA level in peripheral blood mononuclear cell (PBMC) and decidual natural kill (NK) cells.

Product Category

 Recombinant Protein

Protein

 KIR3DL3

Synonyms

 CD158Z; KIR2DS2; KIR3DL7; KIR44; KIRC1; KIR3DL3

Accession

Species

 Human

Biotinylated

 yes

Amino Acid Range

 Glu26-Leu322

Molecular Weight

 The protein has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.

Activity

 The affinity constant of 0.254 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

 C-His-Avi

Expression System

 HEK293

Purity

 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM NaCl, 0.2M L-arginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human B7-H7, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human KIR3DL3, His-Avi Tag with an affinity constant of 0.254 uM as determined in SPR assay (Biacore T200). Human B7-H7, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human KIR3DL3, His-Avi Tag with an affinity constant of 0.254 uM as determined in SPR assay (Biacore T200).
Biotinylated Human KIR3DL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human KIR3DL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human KIR3DL3 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human KIR3DL3 is greater than 95% as determined by SEC-HPLC.

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