Name: Biotinylated Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer Protein (MHC-HM420B)
Brand: KactusBio
SKU: MHC-HM420B-100UG
Price: 310.0 USD
Availability: InStock

Biotinylated Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer Protein (MHC-HM420B)

Immobilized HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) TCR at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag with the EC50 of 0.32ug/ml determined by ELISA.

Biotinylated Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) TCR at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag with the EC50 of 0.32ug/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gly25-Thr305(HLA-A*11:01),Ile21-Met119(B2M)and VVVGADGVGK peptide

Catalog #MHC-HM420B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human HLA-A11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A11:01),Ile21-Met119(B2M) and VVVGADGVGK peptide.
Product Background	Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail.
Product Category	Recombinant Protein / Antibody discovery / MHC

Protein	HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer
Synonyms	MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD
MHC Form	Monomer
Allele	HLA-A*11:01
Accession	AAV53343.1(HLA-A*11:01)&P61769(B2M)&VVVGADGVGK
Species	Human
Biotinylated	yes
Amino Acid Range	Gly25-Thr305(HLA-A*11:01),Ile21-Met119(B2M)and VVVGADGVGK peptide
Molecular Weight	The protein has a predicted MW of 50.3 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized HLA-A11:01&B2M&KRAS G12D (VVVGADGVGK) TCR at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human HLA-A11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag with the EC50 of 0.32ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized HLA-A11:01&B2M&KRAS G12D (VVVGADGVGK) TCR at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human HLA-A11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag with the EC50 of 0.32ug/ml determined by ELISA.
	Biotinylated Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer is greater than 95% as determined by SEC-HPLC.