Biotinylated Human HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) Monomer Protein (MHC-HM422B)
Biotinylated Human HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) Monomer Protein (MHC-HM422B)
Biotinylated Human HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) Monomer Protein (MHC-HM422B)
Biotinylated Human HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) Monomer Protein (MHC-HM422B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 55.67 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and VVGAVGVGK peptide
Catalog #MHC-HM422B
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Product Description
 Recombinant Biotinylated Human HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and VVGAVGVGK peptide.
Product Background
 Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail.
Product Category
 Recombinant Protein
Target
 HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) Monomer
Synonyms
 MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD
MHC Form
 Monomer
Allele
 HLA-A*11:01
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and VVGAVGVGK peptide
Molecular Weight
 The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 56-65 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 55.67 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) Monomer, His Tag captured on CM5 Chip via Anti-His Antibody can bind HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) TCR with an affinity constant of 55.67 uM as determined in SPR assay (Biacore T200).
Biotinylated Human HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*11:01&B2M&KRAS G12V (VVGAVGVGK) Monomer is greater than 95% as determined by SEC-HPLC.

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