Biotinylated Human KRAS WT (HLA-A*03:01) Protein (MHC-HM423B)
Catalog: MHC-HM423B
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| Product Description | Recombinant Biotinylated Human KRAS WT(HLA-A*03:01) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and VVVGAGGVGK peptide. |
| Product Category | Recombinant Protein |
| Target | KRAS WT (HLA-A*03:01) |
| Synonyms | MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD |
| Gene/Protein ID | NP_002107.3(HLA-A*03:01)&P61769(B2M)&VVVGAGGVGK |
| Species | Human |
| Exact Sequence | Gly25-Thr305(HLA-A*03:01) & Ile21-Met119(B2M)and VVVGAGGVGK peptide |
| Molecular Weight | The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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The purity of Biotinylated Human KRAS WT (HLA-A*03:01) is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human KRAS WT (HLA-A*03:01) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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