Biotinylated Human HLA-A*11:01&B2M&KRAS WT (VVVGAGGVGK) Monomer Protein (MHC-HM429B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Exact Sequence: Gly25-Thr305(HLA-A*11:01),Ile21-Met119(B2M)and VVVGAGGVGK peptide

Catalog: MHC-HM429B

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Product Description Recombinant Biotinylated Human HLA-A*11:01&B2M&KRAS WT (VVVGAGGVGK) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*11:01),Ile21-Met119(B2M) and VVVGAGGVGK peptide.

Product Background

Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target.

Product Category

Recombinant Protein

Target

HLA-A*11:01&B2M&KRAS WT (VVVGAGGVGK) Monomer
MHC Form Monomer
Allele HLA-A*11:01

Gene/Protein ID

AAV53343.1(HLA-A*11:01)&P61769(B2M)&VVVGAGGVGK

Species

Human

Exact Sequence

Gly25-Thr305(HLA-A*11:01),Ile21-Met119(B2M)and VVVGAGGVGK peptide
Molecular Weight The protein has a predicted MW of 50.30 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Tris-Bis PAGE result.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The purity of Biotinylated Human HLA-A*11:01&B2M&KRAS WT (VVVGAGGVGK) Monomer is greater than 95% as determined by SEC-HPLC.
Biotinylated Human HLA-A*11:01&B2M&KRAS WT (VVVGAGGVGK) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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