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Biotinylated Human LAG3/CD223 Protein (LAG-HM431B) Summary
Product Description |
Recombinant Biotinylated Human LAG3/CD223 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu23-Gly434. |
Product Background |
LAG-3, is a protein which in humans is encoded by the LAG3 gene, which is a cell surface molecule with diverse biologic effects on T cell function. It is an immune checkpoint receptor and as such is the target of various drug development programs by pharmaceutical companies seeking to develop new treatments for cancer and autoimmune disorders. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Biotinylated Human LAG3/CD223 Protein (LAG-HM431B) Specifications/Details
Protein |
LAG3/CD223 |
Synonyms |
CD223; FDC; LAG3; LAG-3; sLAG-3 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Leu23-Gly434 |
Molecular Weight |
The protein has a predicted MW of 47.3 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human LAG3, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human FGL1, hFc Tag (Cat. FGL-HM211) with the EC50 of 0.16ug/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM PB, 150mM NaCl (pH 6.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human LAG3, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human FGL1, hFc Tag (Cat. FGL-HM211) with the EC50 of 0.16ug/ml determined by ELISA. |
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Immobilized Biotinylated Human LAG3, His-Avi Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-LAG3 Antibody, hFc Tag with the EC50 of 17.5ng/ml determined by ELISA. |
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Biotinylated Human LAG3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human LAG3 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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