Name: Biotinylated Human LAG3/CD223 Protein (LAG-HM431B)
Brand: KactusBio
SKU: LAG-HM431B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human LAG3/CD223 Protein (LAG-HM431B)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Anti-LAG3 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human LAG3, His Tag with the EC50 of 12.7ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Leu23-Gly434

Catalog #LAG-HM431B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human LAG3/CD223 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu23-Gly434.
Product Background	LAG-3, is a protein which in humans is encoded by the LAG3 gene, which is a cell surface molecule with diverse biologic effects on T cell function. It is an immune checkpoint receptor and as such is the target of various drug development programs by pharmaceutical companies seeking to develop new treatments for cancer and autoimmune disorders.
Product Category	Recombinant Protein

Target	LAG3/CD223
Synonyms	CD223; FDC; LAG3; LAG-3; sLAG-3
Accession	P18627-1
Species	Human
Biotinylated	yes
Amino Acid Range	Leu23-Gly434
Molecular Weight	The protein has a predicted MW of 47.3 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-LAG3 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human LAG3, His Tag with the EC50 of 12.7ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Anti-LAG3 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human LAG3, His Tag with the EC50 of 12.7ng/ml determined by ELISA.
	Biotinylated Human LAG3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human LAG3 is greater than 95% as determined by SEC-HPLC.

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