- Express System: HEK293
- Product Tag: C-His-Avi
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Biotinylated Human LAIR1, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-LAIR1 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA (QC Test). See testing image for detail.
- Amino Acid Range: Gln22-His163
Product Description |
Recombinant Biotinylated Human LAIR1/CD305 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln22-His163. |
Product Background |
LAIR1 (leukocyte-associated Ig-like receptor-1, designated CD305) is an approximately 40 kDa type I transmembrane inhibitory glycoprotein belonging to the Ig superfamily.LAIR1 functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
LAIR1/CD305 |
Synonyms |
CD305; HLAIR1; LAIR1; LAIR-1 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gln22-His163 |
Molecular Weight |
The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human LAIR1, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-LAIR1 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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