Product Description |
Recombinant Biotinylated Human LAP (TGF beta 1) Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Leu30-Arg278(C33S). |
Product Background |
Latent TGF beta 1 cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1. Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
LAP (TGF beta 1) |
Synonyms |
CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1; TGF β1; TGFβ; TGF-β-1 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Leu30-Arg278(C33S) |
Molecular Weight |
The protein has a predicted MW of 31.4 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human ITGAV&ITGB6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human LAP (TGF beta 1) , His Tag with the EC50 of 0.11ug/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
N-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
More from Antibody Drug Discovery Proteins
Recently viewed