Biotinylated Human Latent GDF-8 Protein (GDF-HM408B)
  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human Latent GDF-8, His Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 25.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asn24-Ser375
Catalog #GDF-HM408B
Regular price $578.00
/
Size

Order now, ships by:

Product Description

Recombinant Biotinylated Human Latent GDF-8 Protein is expressed from HEK293 with His tag and Avi Tag at the N-terminus.It contains Asn24-Ser375.

Product Background

Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

Latent GDF-8

Synonyms

Myostatin; GDF8; Latent GDF8; Mstn

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Asn24-Ser375

Molecular Weight

The protein has a predicted MW of 43 kDa. Due to glycosylation, the protein migrates to 14 kDa, 38-40 kDa and 45-52 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human Latent GDF-8, His Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 25.9ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human Latent GDF-8, His Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 25.9ng/ml determined by ELISA. Immobilized Biotinylated Human Latent GDF-8, His Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 25.9ng/ml determined by ELISA.
Biotinylated Human Latent GDF-8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human Latent GDF-8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Latent GDF-8 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human Latent GDF-8 is greater than 95% as determined by SEC-HPLC.

Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recently viewed