Biotinylated Human Latent TGF beta 1/TGFB1 Protein (TG1-HM401B)
Catalog: TG1-HM401B
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| Product Description | Recombinant Biotinylated Human Latent TGF beta 1/TGFB1 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal.; It contains Leu30-Ser390 (C33S). |
| Product Category | Recombinant Protein |
| Target | Latent TGF beta 1/TGFB1 |
| Synonyms | CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1 |
| Gene/Protein ID | P01137 |
| Species | Human |
| Exact Sequence | Leu30-Ser390 (C33S) |
| Molecular Weight | The protein has a predicted MW of 44.2 kDa. Due to glycosylation, the protein migrates to 40-50 (LAP) /13-15 (Mature TGF beta 1) kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human ITGAV&ITGB6, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 65.7ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | N-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE; > 93% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human ITGAV&ITGB6, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 65.7ng/ml determined by ELISA. |
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Immobilized Human ITGAV&ITGB8, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 0.39ug/ml determined by ELISA. |
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The purity of Biotinylated Human Latent TGF beta 1 is greater than 93% as determined by SEC-HPLC. |
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Biotinylated Human Latent TGF beta 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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