Biotinylated Human LDLR Protein (LDL-HM401B)
Catalog: LDL-HM401B
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| Product Description | Recombinant Biotinylated Human LDLR Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Ala22-Arg788. |
| Product Category | Recombinant Protein |
| Target | LDLR |
| Synonyms | LDLR; FHC; LDL R; LDL receptor; LDLCQ2; FH |
| Gene/Protein ID | P01130-1 |
| Species | Human |
| Exact Sequence | Ala22-Arg788 |
| Molecular Weight | The protein has a predicted MW of 87.6 kDa, Due to glycosylation, the protein migrates to 110-130 kDa based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 0.23 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Biotinylated Human LDLR, His Tag immobilized on CM5 Chip can bind Human PCSK9, His Tag with an affinity constant of 0.23 nM as determined in SPR assay (Biacore T200). |
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The purity of Biotinylated Human LDLR is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human LDLR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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