Biotinylated Human LDLR Protein (LDL-HM401B)
Biotinylated Human LDLR Protein (LDL-HM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: The affinity constant of 0.23 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ala22-Arg788
Catalog #LDL-HM401B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human LDLR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala22-Arg788.
Product Background
The low density lipoprotein receptor (LDLR) is the founding member of the LDL R family of widely expressed cell surface scavenger receptors. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles.
Product Category
Recombinant Protein
Target
LDLR
Synonyms
LDLR; FHC; LDL R; LDL receptor; LDLCQ2; FH
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Ala22-Arg788
Molecular Weight
The protein has a predicted MW of 87.6 kDa, Due to glycosylation, the protein migrates to 120-140 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 0.23 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human LDLR, His Tag immobilized on CM5 Chip can bind Human PCSK9, His Tag with an affinity constant of 0.23 nM as determined in SPR assay (Biacore T200).
Biotinylated Human LDLR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human LDLR is greater than 95% as determined by SEC-HPLC.
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