- Express System: HEK293
- Product Tag: C-His-Avi
- Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
- Activity: The affinity constant of 0.23 nM as determined in SPR assay (Biacore T200). See testing image for detail.
- Amino Acid Range: Ala22-Arg788
Product Description |
Recombinant Biotinylated Human LDLR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala22-Arg788. |
Product Background |
The low density lipoprotein receptor (LDLR) is the founding member of the LDL R family of widely expressed cell surface scavenger receptors. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
LDLR |
Synonyms |
LDLR; FHC; LDL R; LDL receptor; LDLCQ2; FH |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ala22-Arg788 |
Molecular Weight |
The protein has a predicted MW of 87.6 kDa, Due to glycosylation, the protein migrates to 120-140 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.23 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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