Biotinylated Human LILRB2/CD85d/ILT4 Protein (LIL-HM4B2B)
Biotinylated Human LILRB2/CD85d/ILT4 Protein (LIL-HM4B2B)
Biotinylated Human LILRB2/CD85d/ILT4 Protein (LIL-HM4B2B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human LILRB2, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln22-His458
Catalog #LIL-HM4B2B
Regular price $525.00
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Product Description

Recombinant Biotinylated Human LILRB2/CD85d/ILT4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln22-His458.

Product Background

The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC). LILRB2 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

LILRB2/CD85d/ILT4

Synonyms

CD85d; ILT4; ILT-4; ILT4CD85d; LILRB2; LIR2; MIR10

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Gln22-His458

Molecular Weight

The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human LILRB2, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human LILRB2, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. Immobilized Biotinylated Human LILRB2, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
Biotinylated Human LILRB2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human LILRB2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human LILRB2 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human LILRB2 is greater than 95% as determined by SEC-HPLC.

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