Biotinylated Human LILRB3/CD85a/ILT5 Protein (LIL-HM4B3B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human LILRB3, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly24-Glu443

Catalog: LIL-HM4B3B

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Product Description Recombinant Biotinylated Human LILRB3/CD85a/ILT5 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly24-Glu443.
Product Category Recombinant Protein
Target LILRB3/CD85a/ILT5
Synonyms CD85a; ILT5; ILT-5; LILRB3; LIR3; LIR3CD85A; LIR-3MGC138403; PIRB; HL9
Gene/Protein ID O75022-1
Species Human
Exact Sequence Gly24-Glu443
Molecular Weight The protein has a predicted MW of 50 kDa. Due to glycosylation, the protein migrates to 60-72 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human LILRB3, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human LILRB3, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA.
The purity of Biotinylated Human LILRB3 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human LILRB3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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