Biotinylated Human LILRB3/CD85a/ILT5 Protein (LIL-HM4B3B)
Biotinylated Human LILRB3/CD85a/ILT5 Protein (LIL-HM4B3B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human LILRB3, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly24-Glu443
Catalog #LIL-HM4B3B
Regular price $525.00
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Product Description

Recombinant Biotinylated Human LILRB3/CD85a/ILT5 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly24-Glu443.

Product Background

Leukocyte immunoglobulin-like receptor subfamily B (LILRB3), also known as ILT5, LIR3, and CD85a, is an immunoglobulin superfamily member that is involved in immune regulation. Subfamily B members have cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP-1.LILRB3 may act as receptor for class I MHC antigens. Becomes activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

LILRB3/CD85a/ILT5

Synonyms

CD85a; ILT5; ILT-5; LILRB3; LIR3; LIR3CD85A; LIR-3MGC138403; PIRB; HL9

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Gly24-Glu443

Molecular Weight

The protein has a predicted MW of 50 kDa. Due to glycosylation, the protein migrates to 60-72 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human LILRB3, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human LILRB3, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA. Immobilized Biotinylated Human LILRB3, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA.
Biotinylated Human LILRB3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human LILRB3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human LILRB3 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human LILRB3 is greater than 95% as determined by SEC-HPLC.

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