Name: Biotinylated Human LILRB3/CD85a/ILT5 Protein (LIL-HM4B3B)
Brand: KactusBio
SKU: LIL-HM4B3B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human LILRB3/CD85a/ILT5 Protein (LIL-HM4B3B)

Immobilized Biotinylated Human LILRB3, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.

Biotinylated Human LILRB3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human LILRB3 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human LILRB3, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.
Amino Acid Range: Gly24-Glu443

Catalog #LIL-HM4B3B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human LILRB3/CD85a/ILT5 Protein (LIL-HM4B3B) Summary

Product Description	Recombinant Biotinylated Human LILRB3/CD85a/ILT5 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly24-Glu443.
Product Background	Leukocyte immunoglobulin-like receptor subfamily B (LILRB3), also known as ILT5, LIR3, and CD85a, is an immunoglobulin superfamily member that is involved in immune regulation. Subfamily B members have cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP-1.LILRB3 may act as receptor for class I MHC antigens. Becomes activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Biotinylated Human LILRB3/CD85a/ILT5 Protein (LIL-HM4B3B) Specifications/Details

Protein	LILRB3/CD85a/ILT5
Synonyms	CD85a; ILT5; ILT-5; LILRB3; LIR3; LIR3CD85A; LIR-3MGC138403; PIRB; HL9
Accession	O75022-1
Species	Human
Biotinylated	yes
Amino Acid Range	Gly24-Glu443
Molecular Weight	The protein has a predicted MW of 50 kDa. Due to glycosylation, the protein migrates to 60-72 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human LILRB3, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human LILRB3, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.
	Biotinylated Human LILRB3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human LILRB3 is greater than 95% as determined by SEC-HPLC.