Name: Biotinylated Human LILRB4/CD85k/ILT3 Protein (LIL-HM4B4B)
Brand: KactusBio
SKU: LIL-HM4B4B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human LILRB4/CD85k/ILT3 Protein (LIL-HM4B4B)

Immobilized Anti-LILRB4 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human LILRB4, His Tag with the EC50 of 12.8ng/ml determined by ELISA.

Biotinylated Human LILRB4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human LILRB4 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Anti-LILRB4 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human LILRB4, His Tag with the EC50 of 12.8ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gln22-His257

Catalog #LIL-HM4B4B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human LILRB4/CD85k/ILT3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln22-His257.
Product Background	LILRB4,also known as CD85k and LIR-5, ILT3,- is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	LILRB4/CD85k/ILT3
Synonyms	HM18; ILT3; ILT-3; LILRB4; LIR5; CD85K
Accession	AAH26309
Species	Human
Biotinylated	yes
Amino Acid Range	Gln22-His257
Molecular Weight	The protein has a predicted MW of 28.6 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-LILRB4 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human LILRB4, His Tag with the EC50 of 12.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Anti-LILRB4 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human LILRB4, His Tag with the EC50 of 12.8ng/ml determined by ELISA.
	Biotinylated Human LILRB4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human LILRB4 is greater than 95% as determined by SEC-HPLC.