Product Description |
Recombinant Biotinylated Human LILRB4/CD85k/ILT3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln22-His257. |
Product Background |
LILRB4,also known as CD85k and LIR-5, ILT3,- is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. |
Product Category |
Recombinant Protein |
Target |
LILRB4/CD85k/ILT3 |
Synonyms |
HM18; ILT3; ILT-3; LILRB4; LIR5; CD85K |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gln22-His257 |
Molecular Weight |
The protein has a predicted MW of 28.6 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Anti-LILRB4 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human LILRB4, His Tag with the EC50 of 12.8ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-LILRB4 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human LILRB4, His Tag with the EC50 of 12.8ng/ml determined by ELISA. |
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Biotinylated Human LILRB4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human LILRB4 is greater than 95% as determined by SEC-HPLC. |
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