Biotinylated Human LILRB4/CD85k/ILT3 Protein (LIL-HM4B4B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-LILRB4 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human LILRB4, His Tag with the EC50 of 7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln22-His257

Catalog: LIL-HM4B4B

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Product Description Recombinant Biotinylated Human LILRB4/CD85k/ILT3 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gln22-His257.
Product Category Recombinant Protein
Target LILRB4/CD85k/ILT3
Synonyms HM18; ILT3; ILT-3; LILRB4; LIR5; CD85K
Gene/Protein ID AAH26309
Species Human
Exact Sequence Gln22-His257
Molecular Weight The protein has a predicted MW of 28.6 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-LILRB4 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human LILRB4, His Tag with the EC50 of 7ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-LILRB4 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human LILRB4, His Tag with the EC50 of 7ng/ml determined by ELISA.
The purity of Biotinylated Human LILRB4 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human LILRB4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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