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Biotinylated Human LPAR1 Nanodisc (LAR-HM10NB) Summary
Product Description |
Recombinant Biotinylated Human LPAR1 Nanodisc is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Val364. |
Product Background |
Lysophosphatidic acid receptor 1 (LPA1) belongs to the G protein-coupled receptor family. The ligand for LPA1 is LPA, the simplest lysophospholipid. The mature form of LPA1 is glycosylated and localized on the plasma membrane. LPA1 is bound to heterotrimetric G proteins and transduces intracellular signaling in response to ligation to LPA. Desensitization of LPA1 negatively regulates LPA1-mediated signaling and the resulting biological functions. |
Product Category |
Recombinant Protein / Antibody discovery / Multiple transmembrane proteins / Nanodisc |
Biotinylated Human LPAR1 Nanodisc (LAR-HM10NB) Specifications/Details
Protein |
LPAR1 |
Synonyms |
LPA receptor 1; LPA-1; EDG2; LPA1; Gpcr26 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Met1-Val364 |
Molecular Weight |
The protein has a predicted MW of 54.10 kDa. |
Activity |
Immobilized Biotinylated Human LPAR1 Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LPAR1 Antibody, hFc Tag with the EC50 of 17.6ng/ml determined by ELISA. The affinity constant of 0.13 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). |
Stability And Storage |
Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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|
Biotinylated Human LPAR1 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-LPAR1 Antibody, hFc Tag with an affinity constant of 0.13 uM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Biotinylated Human LPAR1 Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LPAR1 Antibody, hFc Tag with the EC50 of 17.6ng/ml determined by ELISA. |
Other Available Proteins:
Finding available proteins...
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