Biotinylated Human LPAR1 Nanodisc (LAR-HM10NB)

Express System: HEK293
Product Tag: C-His
Activity: Immobilized Biotinylated Human LPAR1 Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LPAR1 Antibody, hFc Tag with the EC50 of 17.6ng/ml determined by ELISA. The affinity constant of 0.13 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Met1-Val364

Catalog #LAR-HM10NB

Price

$2,166⁰⁰

Size

Order by 4pm ET for delivery on:

Biotinylated Human LPAR1 Nanodisc (LAR-HM10NB) Summary

Product Description

Recombinant Biotinylated Human LPAR1 Nanodisc is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Val364.

Product Background

Lysophosphatidic acid receptor 1 (LPA1) belongs to the G protein-coupled receptor family. The ligand for LPA1 is LPA, the simplest lysophospholipid. The mature form of LPA1 is glycosylated and localized on the plasma membrane. LPA1 is bound to heterotrimetric G proteins and transduces intracellular signaling in response to ligation to LPA. Desensitization of LPA1 negatively regulates LPA1-mediated signaling and the resulting biological functions.

Biotinylated Human LPAR1 Nanodisc (LAR-HM10NB) Specifications/Details

Protein	LPAR1
Synonyms	LPA receptor 1; LPA-1; EDG2; LPA1; Gpcr26
Accession	Q92633-1
Species	Human
Biotinylated	yes
Amino Acid Range	Met1-Val364
Molecular Weight	The protein has a predicted MW of 54.10 kDa.
Activity	Immobilized Biotinylated Human LPAR1 Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LPAR1 Antibody, hFc Tag with the EC50 of 17.6ng/ml determined by ELISA. The affinity constant of 0.13 uM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Biotin-labeled
Expression System	HEK293
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Stability And Storage	Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Biotinylated Human LPAR1 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-LPAR1 Antibody, hFc Tag with an affinity constant of 0.13 uM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human LPAR1 Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LPAR1 Antibody, hFc Tag with the EC50 of 17.6ng/ml determined by ELISA.