Biotinylated Human LY6G6D Protein (LYD-HM4GDB)
|Recombinant Biotinylated Human LY6G6D Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Asn20-Ser104.
|LY6G6D is a selectively expressed colorectal cancer antigen that can be used for targeting a therapeutic T-cell response by a T-cell engager.LY6G6D was identified as a selectively expressed CRC antigen that can be utilized to potently re-direct and activate cytotoxic T-cells to lyse LY6G6D expressing CRC using a TcE. This effect can be spread to target negative neighboring tumor cells, potentially leading to improved therapeutic efficacy.
|The protein has a predicted MW of 12.79 kDa. Due to glycosylation, the protein migrates to 15-20 kDa based on Tris-Bis PAGE result.
|Immobilized Biotinylated Human LY6G6D, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LY6G6D Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
|> 95% as determined by Tris-Bis PAGE
|Less than 1EU per ug by the LAL method.
|Shipped at ambient temperature.
|Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
|Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
|Stability And Storage
|Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.