Biotinylated Human LY6G6D Protein (LYD-HM4GDB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Human LY6G6D, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LY6G6D Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asn20-Ser104
Catalog #LYD-HM4GDB
Regular price $577.50
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Biotinylated Human LY6G6D Protein (LYD-HM4GDB) Summary

Product Description

Recombinant Biotinylated Human LY6G6D Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Asn20-Ser104.

Product Background

LY6G6D is a selectively expressed colorectal cancer antigen that can be used for targeting a therapeutic T-cell response by a T-cell engager.LY6G6D was identified as a selectively expressed CRC antigen that can be utilized to potently re-direct and activate cytotoxic T-cells to lyse LY6G6D expressing CRC using a TcE. This effect can be spread to target negative neighboring tumor cells, potentially leading to improved therapeutic efficacy.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Biotinylated Human LY6G6D Protein (LYD-HM4GDB) Specifications/Details

Protein
LY6G6D
Synonyms
Protein Ly6-D; C6orf23; G6D; MEGT1; NG25
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Asn20-Ser104
Molecular Weight
The protein has a predicted MW of 12.79 kDa. Due to glycosylation, the protein migrates to 15-20 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human LY6G6D, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LY6G6D Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human LY6G6D, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LY6G6D Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. Immobilized Biotinylated Human LY6G6D, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LY6G6D Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
Biotinylated Human LY6G6D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human LY6G6D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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