Biotinylated Human LY6G6D Protein (LYD-HM4GDB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Human LY6G6D, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LY6G6D Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asn20-Ser104
Catalog #LYD-HM4GDB
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Product Description
 Recombinant Biotinylated Human LY6G6D Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Asn20-Ser104.
Product Background
 LY6G6D is a selectively expressed colorectal cancer antigen that can be used for targeting a therapeutic T-cell response by a T-cell engager.LY6G6D was identified as a selectively expressed CRC antigen that can be utilized to potently re-direct and activate cytotoxic T-cells to lyse LY6G6D expressing CRC using a TcE. This effect can be spread to target negative neighboring tumor cells, potentially leading to improved therapeutic efficacy.
Product Category
 Recombinant Protein
Target
 LY6G6D
Synonyms
 Protein Ly6-D; C6orf23; G6D; MEGT1; NG25
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Asn20-Ser104
Molecular Weight
 The protein has a predicted MW of 12.79 kDa. Due to glycosylation, the protein migrates to 15-20 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human LY6G6D, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LY6G6D Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human LY6G6D, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LY6G6D Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
Biotinylated Human LY6G6D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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