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Biotinylated Human LY75/CD205 Protein (Primary Amine Labeling) (LY7-HM105B) Summary
Product Description |
Recombinant Biotinylated Human LY75/CD205 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-terminus.It contains Ser28-Asp1666. |
Product Background |
Lymphocyte antigen 75 (Ly75/CD205) is a surface marker on mitotic germ cells in rainbow trout. In mammals, several cell surface molecular markers have been characterized in order to identify the mitotic germ cells. he expression profile of Ly75 protein was similar to that of the mRNA. Furthermore, identification of various fish homologs of ly75 confirmed that their amino acid sequences are well conserved. Therefore, Ly75 may be appropriate for use as a versatile cell surface marker for mitotic germ cells in fish. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Biotinylated Human LY75/CD205 Protein (Primary Amine Labeling) (LY7-HM105B) Specifications/Details
Protein |
LY75/CD205 |
Synonyms |
Ly-75; DEC-205; CD205; CLEC13B; GP200-MR6 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ser28-Asp1666 |
Molecular Weight |
The protein has a predicted MW of 190.2 kDa. Due to glycosylation, the protein migrates to 230-245 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human LY75, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LY75 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Biotinylated Human LY75, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LY75 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA. |
|
|
Biotinylated Human LY75 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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