Biotinylated Human M-CSF/CSF-1 Protein (CSF-HM401B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human M-CSF R, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human M-CSF, His Tag with the EC50 of 19.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Glu33-Asn190

Catalog: CSF-HM401B

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Product Description Recombinant Biotinylated Human M-CSF/CSF-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu33-Asn190.

Product Background

CSF-1, also known as M-CSF , is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. CSF-1 is also essential for the survival and proliferation of osteoclast progenitors.CSF-1 is cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.

Product Category

Recombinant Protein

Target

M-CSF/CSF-1

Gene/Protein ID

P09603-1

Species

Human

Exact Sequence

Glu33-Asn190
Molecular Weight The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 27-37 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human M-CSF R, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human M-CSF, His Tag with the EC50 of 19.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human M-CSF R, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human M-CSF, His Tag with the EC50 of 19.4ng/ml determined by ELISA.
The purity of Biotinylated Human M-CSF is greater than 95% as determined by SEC-HPLC.
Biotinylated Human M-CSF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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