Biotinylated Human M-CSF/CSF-1 Protein (CSF-HM401B)

Immobilized Biotinylated Human M-CSF, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA.

Biotinylated Human M-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human M-CSF is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human M-CSF, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA.
Amino Acid Range: Glu33-Asn190

Catalog #CSF-HM401B

Price

$990⁰⁰

Size

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Biotinylated Human M-CSF/CSF-1 Protein (CSF-HM401B) Summary

Product Description

Recombinant Biotinylated Human M-CSF/CSF-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu33-Asn190.

Product Background

CSF-1, also known as M-CSF , is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. CSF-1 is also essential for the survival and proliferation of osteoclast progenitors.CSF-1 is cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.

Biotinylated Human M-CSF/CSF-1 Protein (CSF-HM401B) Specifications/Details

Protein	M-CSF/CSF-1
Synonyms	CSF1; CSF-1; MCSF; M-CSF; MGC31930; Lanimostim;Colony stimulating factor-1; Colony stimulating factor 1
Accession	P09603-1
Species	Human
Biotinylated	yes
Amino Acid Range	Glu33-Asn190
Molecular Weight	The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 27-37 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human M-CSF, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA.
Product Tag	C-His-Avi
Fluorophore	Biotin-labeled
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human M-CSF, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA.
	Biotinylated Human M-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human M-CSF is greater than 95% as determined by SEC-HPLC.