Biotinylated Human M-CSF R/CSF1R/CD115 Protein (CSF-HM41RB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human M-CSF R , His Tag at 0.5ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-M-CSF R Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.81 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ile20-Glu512
Catalog #CSF-HM41RB
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Product Description
 Recombinant Biotinylated Human M-CSF R/CSF1R/CD115 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile20-Glu512.
Product Background
 CSF1R also known as M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region.CSF1R is tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.
Product Category
 Recombinant Protein
Target
 M-CSF R/CSF1R/CD115
Synonyms
 CD115; CSF1R; CSF-1-R; CSFR; FMSFIM2; M-CSF R; MCSFR; M-CSFR; C-FMS; FIM2; FMS; HDLS
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Ile20-Glu512
Molecular Weight
 The protein has a predicted MW of 57.4 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human M-CSF R , His Tag at 0.5ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-M-CSF R Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.81 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human M-CSF R, His-Avi Tag immobilized on CM5 Chip can bind Human M-CSF, No Tag with an affinity constant of 0.81 uM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human M-CSF R , His Tag at 0.5ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-M-CSF R Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test).
Biotinylated Human M-CSF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human M-CSF R is greater than 95% as determined by SEC-HPLC.

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