Product Description |
Recombinant Biotinylated Human MADCAM1 Protein is expressed from HEK293 with His tag and Avi Tag at the C-Terminus.It contains Gln19-Gln317. |
Product Background |
Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) contributes to the recruitment of donor T cells into the mucosal tissues of the recipient after allogeneic hematopoietic stem cell transplantation (aHSCT). The aim of our study was to determine whether selected single nucleotide polymorphisms (SNPs) of the MADCAM1 gene are associated with development of serious complications after aHSCT. |
Product Category |
Recombinant Protein |
Target |
MADCAM1 |
Synonyms |
MAdCAM-1; MADCAM1; MACAM1 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gln19-Gln317 |
Molecular Weight |
The protein has a predicted MW of 34.31 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human MADCAM1, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Biotinylated Human MADCAM1, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test). |
|
|
Biotinylated Human MADCAM1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human MADCAM1 is greater than 95% as determined by SEC-HPLC. |
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