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Biotinylated Human MASP3 Protein (MSP-HM413B) Summary
Product Description |
Recombinant Biotinylated Human MASP3 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ile450-Arg728. |
Product Background |
Mannose-binding lectin-associated serine protease-1 (also named MASP3), a multifunctional serine protease, plays an important role in innate immunity which is capable of activating the lectin pathway of the complement system and also triggering coagulation cascade system. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Biotinylated Human MASP3 Protein (MSP-HM413B) Specifications/Details
Protein |
MASP3 |
Synonyms |
MASP-3; MASP-1; MASP1; RaRF; Serine protease 5; CRARF; CRARF1; PRSS5; |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ile450-Arg728 |
Molecular Weight |
The protein has a predicted MW of 33.46 kDa. Due to glycosylation, the protein migrates to 42-55 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human MASP3, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MASP3 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is > 15000 pmol/min/µg. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in 50mM Tris, 150mM NaCl (pH 7.5). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Biotinylated Human MASP3, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MASP3 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. |
|
|
Biotinylated Human MASP3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Biotinylated Human MASP3 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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