Biotinylated Human MCP-1/CCL2 Protein (MCP-HM401B)
Catalog: MCP-HM401B
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| Product Description | Recombinant Biotinylated Human MCP-1/CCL2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Thr99. |
Product Background |
Monocyte chemotactic protein-1 (MCP-1) (also referred to as chemokine (C-C motif) ligand 2 (CCL2) is expressed by mainly inflammatory cells and endothelial cells. MCP-1 has been reported to play an important role in the pathogenesis of atherosclerosis and considerable evidence supports that the monocyte containing MCPs and macrophage influences the growth of other cell types within the atherosclerotic lesion. This review will focus on the general structure features of MCP-1 and its role in atherosclerosis. |
Product Category |
Recombinant Protein |
Target |
MCP-1/CCL2 |
| Synonyms | C-C motif chemokine 2; CCL2; HC11; MCAF; MCP-1; HSMCR30; MCP1; SCYA2; SMC-CF; GDCF-2 |
Gene/Protein ID |
P13500 |
Species |
Human |
Exact Sequence |
Gln24-Thr99 |
| Molecular Weight | The protein has a predicted MW of 11.5 kDa. Due to glycosylation, the protein migrates to 15-20 kDa based on Tris-Bis PAGE result. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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The purity of Biotinylated Human MCP-1 is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human MCP-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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