Name: Biotinylated Human MDL-1/CLEC5A Protein (CLE-HM45AB)
Brand: KactusBio
SKU: CLE-HM45AB-100UG
Price: 578.0 USD
Availability: InStock

Biotinylated Human MDL-1/CLEC5A Protein (CLE-HM45AB)

Immobilized Biotinylated Human MDL-1, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MDL-1 Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA.

Biotinylated Human MDL-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human MDL-1 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human MDL-1, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MDL-1 Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Pro28-Lys188

Catalog #CLE-HM45AB

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human MDL-1/CLEC5A Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Pro28-Lys188.
Product Background	In C. elegans, increased lifespan in daf-2 insulin/IGF-1 receptor mutants is accompanied by up-regulation of the MDL-1 Mad basic helix-loop-helix leucine zipper transcription factor.MDL-1, like its mammalian orthologs, is an inhibitor of cell proliferation and growth that slows progression of an age-related pathology in C. elegans (uterine tumors). In addition, intestine-limited rescue of mdl-1 increased lifespan but not to wild type levels. Thus, mdl-1 likely acts both in the intestine and the germline to influence age-related mortality.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	MDL-1/CLEC5A
Synonyms	MDL-1; CLECSF5; MDL1; CLEC5A; DAP12-associating lectin 1
Accession	Q9NY25-1
Species	Human
Biotinylated	yes
Amino Acid Range	Pro28-Lys188
Molecular Weight	The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human MDL-1, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MDL-1 Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human MDL-1, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MDL-1 Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA.
	Biotinylated Human MDL-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human MDL-1 is greater than 95% as determined by SEC-HPLC.