• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human MICA, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 59.7ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Glu24-Gln308
Catalog #MIC-HM40AB
Price
Regular price $57750
Regular price Sale price $57750
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Size
Avantor VWR Fisher Scientific

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Biotinylated Human MICA Protein (MIC-HM40AB) Summary

Product Description

 Recombinant Biotinylated Human MICA Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu24-Gln308.

Product Background

 TMICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin.

Biotinylated Human MICA Protein (MIC-HM40AB) Specifications/Details

Protein
 MICA
Synonyms
 FLJ60820; MGC111087; MICA; PERB11.1; DAMA-345G11.2; FLJ36918; FLJ60820; MGC21250;DAMA345G11.2
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Glu24-Gln308
Molecular Weight
 The protein has a predicted MW of 35.8 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human MICA, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 59.7ng/ml determined by ELISA (QC Test).
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human MICA, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 59.7ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human MICA, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 59.7ng/ml determined by ELISA (QC Test).
Immobilized Anti-MICA Antibody, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human MICA, His Tag with the EC50 of 1.7ug/ml determined by ELISA. Immobilized Anti-MICA Antibody, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human MICA, His Tag with the EC50 of 1.7ug/ml determined by ELISA.
Biotinylated Human MICA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human MICA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human MICA is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human MICA is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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