Biotinylated Human MICA Protein (MIC-HM40AB)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human NKG2D, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human MICA, His Tag with the EC50 of 0.32μg/ml determined by ELISA (QC Test). See testing image for detail.
  • Exact Sequence: Glu24-Gln308

Catalog: MIC-HM40AB

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Product Description Recombinant Biotinylated Human MICA Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Glu24-Gln308.
Product Category Recombinant Protein
Target MICA
Synonyms FLJ60820; MGC111087; MICA; PERB11.1; DAMA-345G11.2; FLJ36918; FLJ60820; MGC21250
Gene/Protein ID Q96QC4
Species Human
Exact Sequence Glu24-Gln308
Molecular Weight The protein has a predicted MW of 35.8 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human NKG2D, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human MICA, His Tag with the EC50 of 0.32μg/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human NKG2D, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human MICA, His Tag with the EC50 of 0.32μg/ml determined by ELISA (QC Test).
Immobilized Anti-MICA Antibody, hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human MICA, His Tag with the EC50 of 3.1μg/ml determined by ELISA.
The purity of Biotinylated Human MICA is greater than 95% as determined by SEC-HPLC.
Biotinylated Human MICA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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