Name: Biotinylated Human MICA Protein (MIC-HM40AB)
Brand: KactusBio
SKU: MIC-HM40AB-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human MICA Protein (MIC-HM40AB)

Immobilized Biotinylated Human MICA, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 59.7ng/ml determined by ELISA (QC Test).

Immobilized Anti-MICA Antibody, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human MICA, His Tag with the EC50 of 1.7ug/ml determined by ELISA.

Biotinylated Human MICA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human MICA is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human MICA, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 59.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Glu24-Gln308

Catalog #MIC-HM40AB

Size

100UG

500UG

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Biotinylated Human MICA Protein (MIC-HM40AB) Summary

Product Description	Recombinant Biotinylated Human MICA Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu24-Gln308.
Product Background	TMICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human MICA Protein (MIC-HM40AB) Specifications/Details

Protein	MICA
Synonyms	FLJ60820; MGC111087; MICA; PERB11.1; DAMA-345G11.2; FLJ36918; FLJ60820; MGC21250
Accession	Q96QC4
Species	Human
Biotinylated	yes
Amino Acid Range	Glu24-Gln308
Molecular Weight	The protein has a predicted MW of 35.8 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human MICA, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 59.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human MICA, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 59.7ng/ml determined by ELISA (QC Test).
	Immobilized Anti-MICA Antibody, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human MICA, His Tag with the EC50 of 1.7ug/ml determined by ELISA.
	Biotinylated Human MICA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human MICA is greater than 95% as determined by SEC-HPLC.