Biotinylated Human MICB Protein (MIC-HM40BB)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE
Biotinylated: yes
Activity: Immobilized Anti-MICB Antibody, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human MICB, His Tag with the EC50 of 0.43ug/ml determined by ELISA. The affinity constant of 60.38 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ala23-Gly298

Catalog: MIC-HM40BB

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Product Description	Recombinant Biotinylated Human MICB Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala23-Gly298.
Product Background	MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB.MICB Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
Product Category	Recombinant Protein

Target	MICB
Synonyms	MICB; MIC-B; PERB11.2
Accession	Q29980
Species	Human
Amino Acid Range	Ala23-Gly298
Molecular Weight	The protein has a predicted MW of 34.4 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Anti-MICB Antibody, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human MICB, His Tag with the EC50 of 0.43ug/ml determined by ELISA. The affinity constant of 60.38 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human NKG2D, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human MICB, His Tag with an affinity constant of 60.38 nM as determined in SPR assay (Biacore T200).
	Immobilized Anti-MICB Antibody, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human MICB, His Tag with the EC50 of 0.43ug/ml determined by ELISA.
	Immobilized Biotinylated Human MICB, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test).
	Biotinylated Human MICB on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.