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Biotinylated Human MICB Protein (MIC-HM40BB) Summary
Product Description |
Recombinant Biotinylated Human MICB Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala23-Gly298. |
Product Background |
MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB.MICB Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Biotinylated Human MICB Protein (MIC-HM40BB) Specifications/Details
Protein |
MICB |
Synonyms |
MICB; MIC-B; PERB11.2 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ala23-Gly298 |
Molecular Weight |
The protein has a predicted MW of 34.4 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human MICB, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test). The affinity constant of 60.38 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human NKG2D, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human MICB, His Tag with an affinity constant of 60.38 nM as determined in SPR assay (Biacore T200). |
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Immobilized Biotinylated Human MICB, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test). |
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Immobilized Anti-MICB Antibody, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human MICB, His Tag with the EC50 of 0.43ug/ml determined by ELISA. |
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Biotinylated Human MICB on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
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The purity of Biotinylated Human MICB is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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