Biotinylated Human MICB Protein (MIC-HM40BB)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-MICB Antibody, hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human MICB, His Tag with the EC50 of 0.43μg/ml determined by ELISA (QC Test). The affinity constant of 60.38 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ala23-Gly298

Catalog: MIC-HM40BB

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Product Description Recombinant Biotinylated Human MICB Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Ala23-Gly298.
Product Category Recombinant Protein
Target MICB
Synonyms MICB; MIC-B; PERB11.2
Gene/Protein ID Q29980
Species Human
Exact Sequence Ala23-Gly298
Molecular Weight The protein has a predicted MW of 34.4 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-MICB Antibody, hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human MICB, His Tag with the EC50 of 0.43μg/ml determined by ELISA (QC Test). The affinity constant of 60.38 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human NKG2D, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human MICB, His Tag with an affinity constant of 60.38 nM as determined in SPR assay (Biacore T200).
Immobilized Anti-MICB Antibody, hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human MICB, His Tag with the EC50 of 0.43μg/ml determined by ELISA (QC Test).
The purity of Biotinylated Human MICB is greater than 95% as determined by SEC-HPLC.
Biotinylated Human MICB on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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