Product Description |
Recombinant Biotinylated Human Midkine Protein is expressed from E.coli with His tag and Avi Tag at the N-Terminus.It contains Val21-Asp143. |
Product Background |
Midkine is a heparin-binding growth factor, originally reported as the product of a retinoic acid-responsive gene during embryogenesis, but currently viewed as a multifaceted factor contributing to both normal tissue homeostasis and disease development. Midkine is abnormally expressed at high levels in various human malignancies and acts as a mediator for the acquisition of critical hallmarks of cancer, including cell growth, survival, metastasis, migration, and angiogenesis. |
Product Category |
Recombinant Protein |
Target |
Midkine |
Synonyms |
MK; ARAP; MDK; MEK; MK1; MKARAP; NEGF2 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Val21-Asp143 |
Molecular Weight |
The protein has a predicted MW of 16.46 kDa same as Bis-Tris PAGE result. |
Product Tag |
N-His-Avi |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Biotinylated Human Midkine on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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