Name: Biotinylated Human MMP-9 Protein (MMP-HM409B)
Brand: KactusBio
SKU: MMP-HM409B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human MMP-9 Protein (MMP-HM409B)

Immobilized Anti-MMP-9 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human MMP-9, His Tag with the EC50 of 32.1ng/ml determined by ELISA.

Biotinylated Human MMP-9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human MMP-9 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Anti-MMP-9 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human MMP-9, His Tag with the EC50 of 32.1ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Ala20-Asp707

Catalog #MMP-HM409B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human MMP-9 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala20-Asp707.
Product Background	Matrix metalloproteinase 9 (MMP9) contributes to this process and deficiencies in the MMP9 lead to impaired healing. Inappropriate expression of MMP9 also contributes to impaired re-epithelialization. Previously we demonstrated that FOXO1 was activated in wound healing but to higher levels in diabetic wounds. To address mechanisms of impaired re-epithelialization we examined MMP9 expression in vivo in full thickness dermal scalp wounds created in experimental K14.
Product Category	Recombinant Protein / Antibody discovery

Protein	MMP-9
Synonyms	MMP-9; GELB; CLG4B; MANDP2; Gelatinase B
Accession	P14780
Species	Human
Biotinylated	yes
Amino Acid Range	Ala20-Asp707
Molecular Weight	The protein has a predicted MW of 79.3 kDa. Due to glycosylation, the protein migrates to 95-105 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-MMP-9 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human MMP-9, His Tag with the EC50 of 32.1ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Anti-MMP-9 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human MMP-9, His Tag with the EC50 of 32.1ng/ml determined by ELISA.
	Biotinylated Human MMP-9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human MMP-9 is greater than 95% as determined by SEC-HPLC.