Biotinylated Human MMP-9 Protein (MMP-HM409B)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Biotinylated: yes
Activity: Immobilized Anti-MMP-9 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human MMP-9, His Tag with the EC50 of 14.3ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ala20-Asp707

Catalog: MMP-HM409B

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Product Description	Recombinant Biotinylated Human MMP-9 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala20-Asp707.
Product Background	Matrix metalloproteinase 9 (MMP9) contributes to this process and deficiencies in the MMP9 lead to impaired healing. Inappropriate expression of MMP9 also contributes to impaired re-epithelialization. Previously we demonstrated that FOXO1 was activated in wound healing but to higher levels in diabetic wounds. To address mechanisms of impaired re-epithelialization we examined MMP9 expression in vivo in full thickness dermal scalp wounds created in experimental K14.
Product Category	Recombinant Protein

Target	MMP-9
Synonyms	MMP-9; GELB; CLG4B; MANDP2; Gelatinase B
Gene/Protein ID	P14780
Species	Human
Exact Sequence	Ala20-Asp707
Molecular Weight	The protein has a predicted MW of 79.3 kDa. Due to glycosylation, the protein migrates to 95-105 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Anti-MMP-9 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human MMP-9, His Tag with the EC50 of 14.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Anti-MMP-9 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human MMP-9, His Tag with the EC50 of 14.3ng/ml determined by ELISA.
	The purity of Biotinylated Human MMP-9 is greater than 95% as determined by SEC-HPLC.
	Biotinylated Human MMP-9 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.