• Express System: HEK293
  • Product Tag: C-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human/Cynomolgus Activin RIIB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Mouse/Rat GDF-8, Avi Tag with the EC50 of 2.5ng/ml determined by ELISA.
  • Amino Acid Range: Asp267-Ser375 (Human/Cynomolgus) / Asp268-Ser376 (Mouse/Rat)
Catalog #GDF-HM008B
Price
Regular price $99000
Regular price Sale price $99000
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human/Cynomolgus/Mouse/Rat GDF-8 Protein (GDF-HM008B) Summary

Product Description

 Recombinant Biotinylated Human/Cynomolgus/Mouse/Rat GDF-8 Protein is expressed from HEK293 with Avi tag at the C-terminus.It contains Asp267-Ser375 (Human/Cynomolgus) / Asp268-Ser376 (Mouse/Rat).

Product Background

 Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain.

Biotinylated Human/Cynomolgus/Mouse/Rat GDF-8 Protein (GDF-HM008B) Specifications/Details

Protein
 GDF-8
Synonyms
 Myostatin; GDF8; Mstn; GDF-8; Myostatin;Growth differentiation factor-8; Growth differentiation factor 8
Accession
Species
 Human/Cynomolgus/Mouse/Rat
Biotinylated
 yes
Amino Acid Range
 Asp267-Ser375 (Human/Cynomolgus) / Asp268-Ser376 (Mouse/Rat)
Molecular Weight
 The protein has a predicted MW of 14.21 kDa. Due to glycosylation, the protein migrates to 15-18 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human/Cynomolgus Activin RIIB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Mouse/Rat GDF-8, Avi Tag with the EC50 of 2.5ng/ml determined by ELISA.
Product Tag
 C-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 4mM HCL. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in 4mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human/Cynomolgus Activin RIIB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Mouse/Rat GDF-8, Avi Tag with the EC50 of 2.5ng/ml determined by ELISA. Immobilized Human/Cynomolgus Activin RIIB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Mouse/Rat GDF-8, Avi Tag with the EC50 of 2.5ng/ml determined by ELISA.
Immobilized Human/Cynomolgus Activin RIIA, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Mouse/Rat GDF-8, Avi Tag with the EC50 of 9.1ng/ml determined by ELISA (QC Test). Immobilized Human/Cynomolgus Activin RIIA, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Mouse/Rat GDF-8, Avi Tag with the EC50 of 9.1ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human/Cynomolgus/Mouse/Rat GDF-8, Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 12.8ng/ml determined by ELISA. Immobilized Biotinylated Human/Cynomolgus/Mouse/Rat GDF-8, Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 12.8ng/ml determined by ELISA.
Biotinylated Human/Cynomolgus/Mouse/Rat GDF-8 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%. Biotinylated Human/Cynomolgus/Mouse/Rat GDF-8 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.
Other Available Proteins:
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