- Express System: HEK293
- Product Tag: C-His-Avi
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Human Nectin-3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Nectin-1, His Tag with the EC50 of 0.52ug/ml determined by ELISA (QC Test). The affinity constant of 22.83 nM as determined in SPR assay (Biacore T200). See testing image for detail.
- Amino Acid Range: Gln31-Thr334
Product Description |
Recombinant Biotinylated Human Nectin-1/PVRL1/CD111 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln31-Thr334. |
Product Background |
Nectins are a small family of Ca -independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions. They are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins.Nectin-1 promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between Nectin-1 and Nectin-3 and between Nectin-1 and Nectin-4. Has some neurite outgrowth-promoting activity. |
Product Category |
Recombinant Protein |
Target |
Nectin-1/PVRL1/CD111 |
Synonyms |
CD111; ED4; HIgRPVRR; HVEC; MGC142031; Nectin1; PRR; PRR1; PRR1MGC16207; PVRL1; PVRR1; SK-12; PVRL1; CLPED1; HIgR; OFC7; PVRR |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gln31-Thr334 |
Molecular Weight |
The protein has a predicted MW of 36.9 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Nectin-3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Nectin-1, His Tag with the EC50 of 0.52ug/ml determined by ELISA (QC Test). The affinity constant of 22.83 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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