Biotinylated Human Nectin-1/PVRL1/CD111 Protein (NEC-HM401B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: The affinity constant of 22.83 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln31-Thr334

Catalog: NEC-HM401B

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Product Description Recombinant Biotinylated Human Nectin-1/PVRL1/CD111 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gln31-Thr334.
Product Category Recombinant Protein
Target Nectin-1/PVRL1/CD111
Synonyms CD111; ED4; HIgRPVRR; HVEC; MGC142031; Nectin1; PRR; PRR1; PRR1MGC16207; PVRL1; PVRR1; SK-12; PVRL1; CLPED1; HIgR; OFC7; PVRR
Gene/Protein ID Q15223-1
Species Human
Exact Sequence Gln31-Thr334
Molecular Weight The protein has a predicted MW of 36.9 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 22.83 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Liquid
Shipping Shipped with blue ice.
Formulation Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
Human Nectin-4, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-1, His-Avi Tag with an affinity constant of 22.83 nM as determined in SPR assay (Biacore T200).
The purity of Biotinylated Human Nectin-1 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Nectin-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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