Name: Biotinylated Human Nectin-2/CD112 Protein (NEC-HM402B)
Brand: KactusBio
SKU: NEC-HM402B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human Nectin-2/CD112 Protein (NEC-HM402B)

Immobilized Biotinylated Human Nectin-2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA (QC Test).

Immobilized BBiotinylated Human Nectin-2, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human PVRIG, mFc Tag with the EC50 of 10.7ng/ml determined by ELISA.

Biotinylated Human Nectin-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human Nectin-2 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human Nectin-2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gln32-Leu360

Catalog #NEC-HM402B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human Nectin-2/CD112 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln32-Leu360.
Product Background	Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. Nectin-2 expression in ovarian cancer may support tumor cell adhesion, leading to growth and lymph node metastasis. Effect of VEGF on Nectin-2 expression as well as permeability was investigated in HUVEC.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	Nectin-2/CD112
Synonyms	CD112; MPH; Nectin2; PRR2; PVRL2; HVEB; PVRR2
Accession	Q92692-2
Species	Human
Biotinylated	yes
Amino Acid Range	Gln32-Leu360
Molecular Weight	The protein has a predicted MW of 38.4 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human Nectin-2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose and 0.01% tween is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human Nectin-2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA (QC Test).
	Immobilized BBiotinylated Human Nectin-2, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human PVRIG, mFc Tag with the EC50 of 10.7ng/ml determined by ELISA.
	Biotinylated Human Nectin-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human Nectin-2 is greater than 95% as determined by SEC-HPLC.