Biotinylated Human Nectin-2/CD112 Protein (NEC-HM402B)
Biotinylated Human Nectin-2/CD112 Protein (NEC-HM402B)
Biotinylated Human Nectin-2/CD112 Protein (NEC-HM402B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human Nectin-2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln32-Leu360
Catalog #NEC-HM402B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human Nectin-2/CD112 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln32-Leu360.
Product Background
Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. Nectin-2 expression in ovarian cancer may support tumor cell adhesion, leading to growth and lymph node metastasis. Effect of VEGF on Nectin-2 expression as well as permeability was investigated in HUVEC.
Product Category
Recombinant Protein
Target
Nectin-2/CD112
Synonyms
CD112; MPH; Nectin2; PRR2; PVRL2; HVEB; PVRR2
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Gln32-Leu360
Molecular Weight
The protein has a predicted MW of 38.4 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human Nectin-2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose and 0.01% tween is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human Nectin-2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA.
Biotinylated Human Nectin-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Nectin-2 is greater than 95% as determined by SEC-HPLC.
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