Name: Biotinylated Human Nectin-3/CD113 Protein (NEC-HM403B)
Brand: KactusBio
SKU: NEC-HM403B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human Nectin-3/CD113 Protein (NEC-HM403B)

Biotinylated Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.13 uM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human Nectin-3, His-Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA (QC Test).

Biotinylated Human Nectin-3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

The purity of Biotinylated Human Nectin-3 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human Nectin-3, His-Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.13 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gly58-Asp400

Catalog #NEC-HM403B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human Nectin-3/CD113 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly58-Asp400.
Product Background	The Nectin family has at least four members (Nectin-1‑4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	Nectin-3/CD113
Synonyms	CD113; CDw113; FLJ90624; PPR3; PVRL3; PVRR3; PRR3
Accession	Q9NQS3-1
Species	Human
Biotinylated	yes
Amino Acid Range	Gly58-Asp400
Molecular Weight	The protein has a predicted MW of 40.6 kDa. Due to glycosylation, the protein migrates to 68-82 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human Nectin-3, His-Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.13 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM PB, 250mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Biotinylated Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.13 uM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human Nectin-3, His-Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA (QC Test).
	Biotinylated Human Nectin-3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	The purity of Biotinylated Human Nectin-3 is greater than 95% as determined by SEC-HPLC.