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Biotinylated Human Nectin-4 Protein, Ultra Low Endotoxin (NEC-HM404B-UL) Summary
Product Description |
Recombinant Biotinylated Human Nectin-4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly32-Val351. |
Product Background |
NECTIN-4 [a poliovirus receptor-related-4 (pvrl-4) encoded protein] is a Ca2+ independent immunoglobulin-like protein. Along with other Nectins (Nectin-1, -2 and -3), it is primarily involved in cell-cell adhesion. In contrast to other Nectins, Nectin-4 is specifically enriched in the embryonic and placental tissues but its expression significantly declines in adult life. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets | ADC targets |
Biotinylated Human Nectin-4 Protein, Ultra Low Endotoxin (NEC-HM404B-UL) Specifications/Details
Protein |
Nectin-4 |
Synonyms |
EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gly32-Val351 |
Molecular Weight |
The protein has a predicted MW of 37.2 kDa. Due to glycosylation, the protein migrates to 46-48 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human Nectin-4, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human Nectin-4, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA. |
|
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Biotinylated Human Nectin-4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
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The purity of Bioyintlated Human Nectin-4 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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