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Biotinylated Human NKG2A/CD159a Protein (NKG-HM410B) Summary
Product Description |
Recombinant Biotinylated Human NKG2A/CD159a Protein is expressed from HEK293 with His tag and Avi tag at N-Terminus.It contains Arg100-Leu233. |
Product Background |
NKG2, also known as NKG2A(CD159A), is a member of the killer cell lectin-like receptor family. NKG2A marks a unique immune effector subset preferentially co-expressing the tissue-resident CD103 molecule, but not immune checkpoint inhibitors.It is expressed only in NK-cells, but not in T-cells or B-cells. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Biotinylated Human NKG2A/CD159a Protein (NKG-HM410B) Specifications/Details
Protein |
NKG2A/CD159a |
Synonyms |
NKG2A; NKG2-A; CD94; KLRD1; KP43; KLRC1; CD159A |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Arg100-Leu233 |
Molecular Weight |
The protein has a predicted MW of 18.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human NKG2A, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA. |
Product Tag |
N-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Biotinylated Human NKG2A, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA. |
|
|
Biotinylated Human NKG2A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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