Biotinylated Human NKG2A&CD94 Protein (NKC-HM495B)
Catalog: NKC-HM495B
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Product Description | Recombinant Biotinylated Human NKG2A&CD94 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal.; It contains Arg100-Leu233 (NKG2A)&Ser34-Ile179 (CD94). |
Product Category | Recombinant Protein |
Target | NKG2A&CD94 |
Synonyms | CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94 |
Gene/Protein ID | P26715-1 (NKG2A)&Q13241-1 (CD94) |
Species | Human |
Exact Sequence | Arg100-Leu233 (NKG2A)&Ser34-Ile179 (CD94) |
Molecular Weight | The protein has a predicted MW of 17.9 kDa (CD94)&18.3 kDa (NKG2A). Due to glycosylation, the protein migrates to 33-48 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Biotinylated Human NKG2A&CD94, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA. See testing image for detail. |
Product Tag | N-His-Avi |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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