Biotinylated Human NKG2A&CD94 Protein (NKC-HM495B)
Biotinylated Human NKG2A&CD94 Protein (NKC-HM495B)
Biotinylated Human NKG2A&CD94 Protein (NKC-HM495B)
  • Express System: HEK293
  • Product Tag: N-His-Avi, N-Flag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human NKG2A&CD94, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)
Catalog #NKC-HM495B
Regular price $525.00
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Product Description

Recombinant Biotinylated Human NKG2A&CD94 Protein is expressed from HEK293 with His tag and Avi tag and Flag tag at the N-Terminus.It contains Arg100-Leu233(NKG2A)&Ser34-Ile179(CD94).

Product Background

The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.

Product Category

Recombinant Protein / Antibody discovery

Protein

NKG2A&CD94

Synonyms

CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)

Molecular Weight

The protein has a predicted MW of 17.9 kDa (CD94)&18.3 kDa (NKG2A). Due to glycosylation, the protein migrates to 33-48 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human NKG2A&CD94, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

N-His-Avi, N-Flag

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human NKG2A&CD94, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human NKG2A&CD94, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test).
Biotinylated Human NKG2A&CD94 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human NKG2A&CD94 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC.

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