Biotinylated Human NKG2A&CD94 Protein (NKC-HM495B)
Biotinylated Human NKG2A&CD94 Protein (NKC-HM495B)
Biotinylated Human NKG2A&CD94 Protein (NKC-HM495B)
  • Express System: HEK293
  • Product Tag: N-His-Avi, N-Flag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human NKG2A&CD94, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)
Catalog #NKC-HM495B
Regular price $577.50
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Biotinylated Human NKG2A&CD94 Protein (NKC-HM495B) Summary

Product Description

Recombinant Biotinylated Human NKG2A&CD94 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus of NKG2A and Flag tag at the N-Terminus of CD94.It contains Arg100-Leu233(NKG2A) and Ser34-Ile179(CD94).

Product Background

The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Biotinylated Human NKG2A&CD94 Protein (NKC-HM495B) Specifications/Details

Protein
NKG2A&CD94
Synonyms
CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)
Molecular Weight
The protein has a predicted MW of 17.9 kDa (CD94)&18.3 kDa (NKG2A). Due to glycosylation, the protein migrates to 33-48 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human NKG2A&CD94, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

N-His-Avi, N-Flag
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human NKG2A&CD94, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human NKG2A&CD94, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test).
Biotinylated Human NKG2A&CD94 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human NKG2A&CD94 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC.
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