Name: Biotinylated Human NKG2A&CD94 Protein (NKC-HM495B)
Brand: KactusBio
SKU: NKC-HM495B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human NKG2A&CD94 Protein (NKC-HM495B)

Immobilized Biotinylated Human NKG2A&CD94, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test).

Biotinylated Human NKG2A&CD94 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Avi, N-Flag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human NKG2A&CD94, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)

Catalog #NKC-HM495B

Size

100UG

500UG

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Biotinylated Human NKG2A&CD94 Protein (NKC-HM495B) Summary

Product Description	Recombinant Biotinylated Human NKG2A&CD94 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus of NKG2A and Flag tag at the N-Terminus of CD94.It contains Arg100-Leu233(NKG2A) and Ser34-Ile179(CD94).
Product Background	The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human NKG2A&CD94 Protein (NKC-HM495B) Specifications/Details

Protein	NKG2A&CD94
Synonyms	CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94
Accession	P26715-1(NKG2A)&Q13241-1(CD94)
Species	Human
Biotinylated	yes
Amino Acid Range	Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)
Molecular Weight	The protein has a predicted MW of 17.9 kDa (CD94)&18.3 kDa (NKG2A). Due to glycosylation, the protein migrates to 33-48 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human NKG2A&CD94, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	N-His-Avi, N-Flag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human NKG2A&CD94, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test).
	Biotinylated Human NKG2A&CD94 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC.