Biotinylated Human NKG2D/CD314 Protein (NKG-HM42DB)

  • Express System: Expi293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human NKG2D, His Tag at 1_g/ml (100_l/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Phe78-Val216

Catalog: NKG-HM42DB

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Product Description Recombinant Biotinylated Human NKG2D/CD314 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal.It contains Phe78-Val216.
Product Category Recombinant Protein
Target NKG2D/CD314
Synonyms CD314; D12S2489E; KLR; NKG2-D; NKG2D
Gene/Protein ID P26718
Species Human
Exact Sequence Phe78-Val216
Molecular Weight The protein has a predicted MW of 19.0 kDa. Due to glycosylation, the protein migrates to 36-40 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human NKG2D, His Tag at 1_g/ml (100_l/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human NKG2D, His Tag at 1_g/ml (100_l/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
The purity of Biotinylated Human NKG2D is greater than 95% as determined by SEC-HPLC.
Biotinylated Human NKG2D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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