Biotinylated Human NKG2D/CD314 Protein (NKG-HM42DB)

  • Express System: Expi293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human NKG2D, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Phe78-Val216

Catalog: NKG-HM42DB

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Product Description Recombinant Biotinylated Human NKG2D/CD314 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal.; It contains Phe78-Val216 .
Product Category Recombinant Protein
Target NKG2D/CD314
Synonyms CD314; D12S2489E; KLR; NKG2-D; NKG2D
Gene/Protein ID P26718
Species Human
Exact Sequence Phe78-Val216
Molecular Weight The protein has a predicted MW of 19.0 kDa. Due to glycosylation, the protein migrates to 36-40 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human NKG2D, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human NKG2D, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
The purity of Biotinylated Human NKG2D is greater than 95% as determined by SEC-HPLC.
Biotinylated Human NKG2D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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