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Biotinylated Human NKG2D/CD314 Protein, Ultra Low Endotoxin (NKG-HM42DB-UL) Summary
Product Description |
Recombinant Biotinylated Human NKG2D/CD314 Protein is expressed from HEK293 with Avi tag and His tag at the N-Terminus.It contains Phe78-Val216. |
Product Background |
NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets |
Biotinylated Human NKG2D/CD314 Protein, Ultra Low Endotoxin (NKG-HM42DB-UL) Specifications/Details
Protein |
NKG2D/CD314 |
Synonyms |
CD314; D12S2489E; KLR; NKG2-D; NKG2D |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Phe78-Val216 |
Molecular Weight |
The protein has a predicted MW of 19.0 kDa. Due to glycosylation, the protein migrates to 36-40 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human NKG2D, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human MICA, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test). |
Product Tag |
N-Avi-His |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human NKG2D, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human MICA, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test). |
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Immobilized Anti-NKG2D Antibody at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NKG2D, His-Avi Tag with the EC50 of 0.39ug/ml determined by ELISA. |
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Biotinylated Human NKG2D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human NKG2D is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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