Biotinylated Human NKp30/NCR3/CD337 Protein (NKP-HM430B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-NKp30 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human NKp30, His Tag with the EC50 of 30ng/ml determined by ELISA. The affinity constant of 0.330 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Leu19-Thr138

Catalog: NKP-HM430B

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Product Description Recombinant Biotinylated Human NKp30/NCR3/CD337 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Leu19-Thr138.
Product Category Recombinant Protein
Target NKp30/NCR3/CD337
Synonyms CD337; LY117; NCR3; NKp30; 1C7; MALS
Gene/Protein ID AAH52582
Species Human
Exact Sequence Leu19-Thr138
Molecular Weight The protein has a predicted MW of 20 kDa. Due to glycosylation, the protein migrates to 26-45 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-NKp30 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human NKp30, His Tag with the EC50 of 30ng/ml determined by ELISA. The affinity constant of 0.330 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human B7-H6, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human NKp30, His-Avi Tag with an affinity constant of 0.330 μM as determined in SPR assay (Biacore T200).
Immobilized Anti-NKp30 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human NKp30, His Tag with the EC50 of 30ng/ml determined by ELISA.
The purity of Biotinylated Human NKp30 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human NKp30 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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