Biotinylated Human NKp30/NCR3/CD337 Protein (NKP-HM430B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human NKp30, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NKp30 Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.330 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Leu19-Thr138

Catalog: NKP-HM430B

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Product Description Recombinant Biotinylated Human NKp30/NCR3/CD337 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu19-Thr138.
Product Category Recombinant Protein
Target NKp30/NCR3/CD337
Synonyms CD337; LY117; NCR3; NKp30; 1C7; MALS
Gene/Protein ID AAH52582
Species Human
Exact Sequence Leu19-Thr138
Molecular Weight The protein has a predicted MW of 20 kDa. Due to glycosylation, the protein migrates to 26-45 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human NKp30, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NKp30 Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.330 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human B7-H6, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human NKp30, His-Avi Tag with an affinity constant of 0.330 uM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human NKp30, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NKp30 Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA (QC Test).
The purity of Biotinylated Human NKp30 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human NKp30 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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