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Biotinylated Human NKp30/NCR3/CD337 Protein (NKP-HM430B) Summary
Product Description |
Recombinant Biotinylated Human NKp30/NCR3/CD337 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu19-Thr138. |
Product Background |
NKp30, along with NKp44 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors". These receptors play a major role in triggering NK-mediated killing of most tumor cells lines.NKp30 stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It controls, for instance, NK cells cytotoxicity against tumor cells. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Biotinylated Human NKp30/NCR3/CD337 Protein (NKP-HM430B) Specifications/Details
Protein |
NKp30/NCR3/CD337 |
Synonyms |
CD337; LY117; NCR3; NKp30; 1C7; MALS |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Leu19-Thr138 |
Molecular Weight |
The protein has a predicted MW of 20 kDa. Due to glycosylation, the protein migrates to 26-45 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human NKp30, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NKp30 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.330 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human B7-H6, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human NKp30, His-Avi Tag with an affinity constant of 0.330 uM as determined in SPR assay (Biacore T200). |
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|
Immobilized Biotinylated Human NKp30, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NKp30 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA (QC Test). |
|
|
Biotinylated Human NKp30 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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