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Biotinylated Human NKp46/NCR1/CD335 Protein (Primary Amine Labeling) (NKP-HM146B) Summary
Product Description |
Recombinant Biotinylated Human NKp46/NCR1/CD335 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-Terminus.It contains Gln22-Asn254. |
Product Background |
NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR).These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Biotinylated Human NKp46/NCR1/CD335 Protein (Primary Amine Labeling) (NKP-HM146B) Specifications/Details
Protein |
NKp46/NCR1/CD335 |
Synonyms |
CD335; Ly94; NCR1; NKp46; MAR-1; NKP46FLJ99094 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gln22-Asn254 |
Molecular Weight |
The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human NKp46, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NKp46 Antibody, hFc Tag with the EC50 of 19.7ng/ml determined by ELISA. |
Product Tag |
N-His |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human NKp46, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NKp46 Antibody, hFc Tag with the EC50 of 19.7ng/ml determined by ELISA. |
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Biotinylated Human NKp46 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human NKp46 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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