Biotinylated Human NPR1/NPRA Protein (NPR-HM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Anti-NPR1 antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NPR1, His Tag with the EC50 of 2.1ug/ml determined by ELISA (QC Test). The affinity constant of 2.34 µM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly33-Glu473
Catalog #NPR-HM401B
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Product Description

 Recombinant Biotinylated Human NPR1/NPRA Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly33-Glu473.

Product Background

 NPR1 (natriuretic peptide receptor 1), a receptor of ANP (atrial natriuretic peptide) whitch acting through NPR1, provokes hypotension. NPR1 was abundantly expressed in endothelial cells and smooth muscle cells of small arteries and arterioles. NPR1 plays a crucial role in ANP-mediated blood pressure regulation, presumably by a mechanism that is RGS2-dependent in the acute phase and RGS2-independent in the chronic phase.

Product Category

 Recombinant Protein

Protein

 NPR1/NPRA

Synonyms

 ANP-A; ANPR-A; NPR-A; GC-A; ANPRA

Accession

Species

 Human

Biotinylated

 yes

Amino Acid Range

 Gly33-Glu473

Molecular Weight

 The protein has a predicted MW of 51.8 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Bis-Tris PAGE result.

Activity

 Immobilized Anti-NPR1 antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NPR1, His Tag with the EC50 of 2.1ug/ml determined by ELISA (QC Test). The affinity constant of 2.34 µM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

 C-His-Avi

Expression System

 HEK293

Purity

 >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC

Endotoxin

 Less than 1EU per ug by the LAL method.
Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Anti-NPR1 Antibody, hFcTag captured on CM5 Chip via Protein A can bind Biotinylated Human NPR1, His Tag with an affinity constant of 2.34 µM as determined in SPR assay (Biacore T200). Anti-NPR1 Antibody, hFcTag captured on CM5 Chip via Protein A can bind Biotinylated Human NPR1, His Tag with an affinity constant of 2.34 µM as determined in SPR assay (Biacore T200).
Immobilized Anti-NPR1 antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NPR1, His Tag with the EC50 of 2.1ug/ml determined by ELISA (QC Test). Immobilized Anti-NPR1 antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NPR1, His Tag with the EC50 of 2.1ug/ml determined by ELISA (QC Test).
Biotinylated Human NPR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human NPR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human NPR1 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human NPR1 is greater than 95% as determined by SEC-HPLC.

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