Biotinylated Human NPR1/NPRA Protein (active dimer) (NPR-HM401B)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Biotinylated Human NPR1, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NPR1 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test). The affinity constant of 2.34 µM as determined in SPR assay (Biacore T200).
Amino Acid Range: Gly33-Glu473

Catalog #NPR-HM401B

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$577.50

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Biotinylated Human NPR1/NPRA Protein (active dimer) (NPR-HM401B) Summary

Product Description	Recombinant Biotinylated Human NPR1/NPRA Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly33-Glu473.
Product Background	NPR1 (natriuretic peptide receptor 1), a receptor of ANP (atrial natriuretic peptide) whitch acting through NPR1, provokes hypotension. NPR1 was abundantly expressed in endothelial cells and smooth muscle cells of small arteries and arterioles. NPR1 plays a crucial role in ANP-mediated blood pressure regulation, presumably by a mechanism that is RGS2-dependent in the acute phase and RGS2-independent in the chronic phase.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Protein	NPR1/NPRA
Synonyms	ANP-A; ANPR-A; NPR-A; GC-A; ANPRA
Accession	NP_000897.3
Species	Human
Biotinylated	yes
Amino Acid Range	Gly33-Glu473
Molecular Weight	The protein has a predicted MW of 51.8 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human NPR1, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NPR1 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test). The affinity constant of 2.34 µM as determined in SPR assay (Biacore T200).
Product Tag	C-His-Avi
Fluorophore	Biotin-labeled
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Anti-NPR1 Ab.2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human NPR1, His Tag with an affinity constant of 2.34 µM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human NPR1, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NPR1 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test).
	Biotinylated Human NPR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human NPR1 is greater than 90% and the molecular weight of this protein is around 110-120 kDa as determined by SEC-MALS.
	Loaded Biotinylated Human NPR1, His-Avi Tag on Streptavidin-Biosensor can bind Anti-NPR1 Ab.2, hFc Tag with an affinity constant of 1.35 nM as determined in BLI assay.
	The purity of Biotinylated Human NPR1 is greater than 90% as determined by SEC-HPLC.

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