- Express System: HEK293
- Product Tag: N-hFc
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Human Her4, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NRG1 Beta 1, hFc Tag with the EC50 of 0.65ug/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Ser2-Lys246
Product Description |
Recombinant Biotinylated Human NRG1 Beta 1 Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the N-terminus.It contains Ser2-Lys246. |
Product Background |
Neuregulin-1 (NRG-1) is a ligand of the epidermal growth factor receptor (erbB), and its interaction involves activation of the glutamatergic N-methyl-Daspartate receptor, which increases the expression of the β2 subunit of the γ- aminobutyric acid receptor and subunits of the nicotinic acetylcholine receptor. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
NRG1 Beta 1 |
Synonyms |
Pro-NRG1; NRG1; GGF; HGL; HRGA; NDF; SMDF; Beta1; Beta1A |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ser2-Lys246 |
Molecular Weight |
The protein has a predicted MW of 54.00 kDa. Due to glycosylation, the protein migrates to 75-90 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Her4, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NRG1 Beta 1, hFc Tag with the EC50 of 0.65ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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