• Express System: HEK293
  • Product Tag: N-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Her4, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NRG1 Beta 1, hFc Tag with the EC50 of 0.65ug/ml determined by ELISA.
  • Amino Acid Range: Ser2-Lys246
Catalog #NRG-HM201B
Price
Regular price $57750
Regular price Sale price $57750
/
Size
Avantor VWR Fisher Scientific

Order by 4pm ET for delivery on:

Biotinylated Human NRG1 Beta 1 Protein (Primary Amine Labeling) (NRG-HM201B) Summary

Product Description

 Recombinant Biotinylated Human NRG1 Beta 1 Protein (Primary Amine Labeling) is expressed from HEK293 with hFc (IgG1) tag at the N-terminus.It contains Ser2-Lys246.

Product Background

 Neuregulin-1 (NRG-1) is a ligand of the epidermal growth factor receptor (erbB), and its interaction involves activation of the glutamatergic N-methyl-Daspartate receptor, which increases the expression of the β2 subunit of the γ- aminobutyric acid receptor and subunits of the nicotinic acetylcholine receptor.

Biotinylated Human NRG1 Beta 1 Protein (Primary Amine Labeling) (NRG-HM201B) Specifications/Details

Protein
 NRG1 Beta 1
Synonyms
 Pro-NRG1; NRG1; GGF; HGL; HRGA; NDF; SMDF; Beta1; Beta1A
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Ser2-Lys246
Molecular Weight
 The protein has a predicted MW of 54.00 kDa. Due to glycosylation, the protein migrates to 75-90 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Her4, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NRG1 Beta 1, hFc Tag with the EC50 of 0.65ug/ml determined by ELISA.
Product Tag
 N-hFc (IgG1)
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human Her4, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NRG1 Beta 1, hFc Tag with the EC50 of 0.65ug/ml determined by ELISA. Immobilized Human Her4, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NRG1 Beta 1, hFc Tag with the EC50 of 0.65ug/ml determined by ELISA.
Biotinylated Human NRG1 Beta 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human NRG1 Beta 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human NRG1 Beta 1 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human NRG1 Beta 1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...