Name: Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer Protein (MHC-HM405B)
Brand: KactusBio
SKU: MHC-HM405B-100UG
Price: 341.0 USD
Availability: InStock

ELISA binding curve for biotinylated recombinant human NY-ESO-1 (HLA-A02:01) complex with His tag, showing interaction with anti-NY-ESO-1 (HLA-A02:01) antibody (hFc tag).

Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer Protein (MHC-HM405B)

Immobilized Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with the EC50 of 73.7 ng/ml determined by ELISA (QC Test).

Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer was greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with the EC50 of 73.7 ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLMWITQC peptide

Catalog #MHC-HM405B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human HLA-A02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305(HLA-A02:01), Ile21-Met119(B2M) and SLLMWITQC peptide.
Product Background	NY-ESO-1 or New York esophageal squamous cell carcinoma 1 is a well-known cancer-testis antigen (CTAs) with re-expression in numerous cancer types. Its ability to elicit spontaneous humoral and cellular immune responses, together with its restricted expression pattern, have rendered it a good candidate target for cancer immunotherapy.
Product Category	Recombinant Protein / Antibody discovery / MHC

Protein	HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer
Synonyms	MHC; MY-ESO-1; CT6.1; LAGE-2; CTAG1; CTAG1B; ESO1CTAG; LAGE2A; NY-ESO-1; CTAG1A
MHC Form	Monomer
Allele	HLA-A*02:01
Accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&SLLMWITQC
Species	Human
Biotinylated	yes
Amino Acid Range	Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLMWITQC peptide
Molecular Weight	The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-HLA-A02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human HLA-A02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with the EC50 of 73.7 ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS, 100 mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Anti-HLA-A02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human HLA-A02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with the EC50 of 73.7 ng/ml determined by ELISA (QC Test).
	Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer was greater than 95% as determined by SEC-HPLC.